A genome-wide association study and transcriptome analysis reveal the genetic basis for the Southern corn rust resistance in maize  

作　　者：Yang Wang Chunhua Mu Xiangdong Li Canxing Duan Jianjun Wang Xin Lu Wangshu Li Zhennan Xu Shufeng Sun Ao Zhang Zhiqiang Zhou Shenghui Wen Zhuanfang Hao Jienan Han Jianzhou Qu Wanli Du Fenghai Li Jianfeng Weng 

机构地区：[1]College of Agronomy,Shenyang Agricultural University,Shenyang 110866,China [2]Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [3]Institute of Maize Research,Shandong Academy of Agricultural Sciences,Jinan 250100,China [4]College of Plant Protection,Shandong Agricultural University,Tai’an 250100,China [5]Institute of Maize,Shanxi Agricultural University,Xinzhou 034000,China

出　　处：《Journal of Integrative Agriculture》2025年第2期453-466,共14页农业科学学报(英文版)

基　　金：supported by the National Key R&D Program of China(2022YFD1201802);the Shandong Key R&D Program,China(2022CXGC010607);the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202109);the Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ASTIP2017-ICS);the Subproject of the Major Project of Science and Technology in Shanxi Province,China(202201140601025-1-02)。

摘　　要：Southern corn rust(SCR)is an airborne fungal disease caused by Puccinia polysora Underw.(P.polysora)that adversely impacts maize quality and yields worldwide.Screening for new elite SCR-resistant maize loci or genes has the potential to enhance overall resistance to this pathogen.Using phenotypic SCR resistance-related data collected over two years and three geographical environments,a genome-wide association study was carried out in this work,which eventually identified 91 loci that were substantially correlated with SCR susceptibility.These included 13 loci that were significant in at least three environments and overlapped with 74 candidate genes(B73_RefGen_v4).Comparative transcriptomic analyses were then performed to identify the genes related to SCR infection,with 2,586 and 797 differentially expressed genes(DEGs)ultimately being identified in the resistant Qi319and susceptible 8112 inbred lines following P.polysora infection,respectively,including 306 genes common to both lines.Subsequent integrative multi-omics investigations identified four potential candidate SCR response-related genes.One of these genes is ZmHCT9,which encodes the protein hydroxycinnamoyl transferase 9.This gene was up-regulated in susceptible inbred lines and linked to greater P.polysora resistance as confirmed through cucumber mosaic virus(CMV)-based virus induced-gene silencing(VIGS)system-mediated gene silencing.These data provide important insights into the genetic basis of the maize SCR response.They will be useful for for future research on potential genes related to SCR resistance in maize.

关 键 词：MAIZE southern corn rust genome-wide association study TRANSCRIPTOME 

分 类 号：S435.131.4[农业科学—农业昆虫与害虫防治]