On-site visual detection of Nipah virus combining a reverse transcription recombinase-aided amplification with a lateral-flow dipstick assay  

作　　者：Kaikai Jin Junjie Zhao Huanxin Chen Zimo Zhang Zengguo Cao Zanheng Huang Hao Li Yongsai Liu Lisi Ai Yufei Liu Changqi Fan Yuanyuan Li Pei Huang Hualei Wang Haili Zhang 

机构地区：[1]State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory for Zoonosis Research of the Ministry of Education/Institute of Zoonosis,College of Veterinary Medicine,Jilin University,Changchun 130062,China [2]Key Laboratory of Special Pathogens and Biosafety,Wuhan Institute of Virology,Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan 430071,China [3]National/WOAH Reference Laboratory for Classical Swine Fever,China Institute of Veterinary Drug Control,Beijing 100081,China [4]Zhili College,Tsinghua University,Beijing 100084,China

出　　处：《Journal of Integrative Agriculture》2025年第2期790-794,共5页农业科学学报(英文版)

基　　金：supported by the National Key Research and Development Program of China(2021YFF0703600)。

摘　　要：Highlights The RT-RAA-VF assay developed for the NiV P gene can perform rapid detection of NiV within 20 min at 42℃ with high specificity.This assay is capable of attaining sensitivity to a single copy of NiV RNA transcripts.This assay effectively avoids false positives caused by aerosol contamination with a sealed disposable nucleic acid visualization test paper device.

关 键 词：Singh destroyed visual 

分 类 号：S852.65[农业科学—基础兽医学]