高效液相色谱法同时测定葵花盘粉提取物中的东莨菪素、芦丁、槲皮素和木樨草素  

Determination of Scopoletin,Rutin,Quercetin and Luteolin in Sunflower Calathide Powder Extractions by Using HPLC

作　　者：巩润梓 吴大刚 邢小霞 李平亮 GONG Run-zi;WU Da-gang;XING Xiao-xia;LI Ping-liang(College of Plant Health and Medicine,Qingdao Agricultural University,Qingdao 266109,China;Shandong Muxiang Biotechnology Co.,Ltd.,Qingdao 266199,China)

机构地区：[1]青岛农业大学植物医学学院,山东青岛266109 [2]山东牧香生物科技有限公司,山东青岛266199

出　　处：《化学试剂》2025年第3期85-90,共6页Chemical Reagents

基　　金：青岛农业大学横向科研项目(6602424282);青岛农业大学高层次人才科研基金项目(6631114318)。

摘　　要：选择东莨菪素、芦丁、槲皮素和木樨草素作为葵花盘粉提取物抗痛风活性成分的代表,通过优化QuEChERS前处理方法的提取液、净化剂组成以及色谱流动相组成、梯度洗脱程序、检测定量波长,建立了同时测定葵花盘粉提取物样品中4种成分的高效液相色谱分析方法。4种化合物的定量限和检出限范围分别为0.08~0.15 mg/L和0.02~0.06 mg/L,添加回收率范围为87%~97%,相对标准偏差范围1.1%~5.2%。采用该方法检测了3种葵花盘粉样品,结果表明东莨菪素、芦丁、槲皮素和木樨草素的含量范围分别为68.75~179.41、48.52~232.35、20.45~32.47和0.34~1.10 mg/kg,为葵花盘粉相关产品的活性成分检测与质量控制提供了方法学参考。Scopoletin,rutin,quercetin and luteolin were selected as the representatives of the active ingredients of sunflower calathide powder extraction against hyperuricemia.A method based on high-performance liquid chromatography(HPLC)for simultaneous determination of four compounds in sunflower calathide was developed by optimizing the extraction solution by QuEChERS method,constituent of purification agents and mobile phases,procedure of gradient elution,and detection wavelength.The limit of detection and limit of quantification were in the range of 0.08~0.15 mg/L and 0.02~0.06 mg/L,respectively.The recovery of four compounds from real samples was in the range of 87%~97%with relative standard deviation(RSD)of 1.1%~5.2%.Then this method was applied to detect four compounds in products of three sunflower calathide powder.The results showed that the concentrations of scopoletin,rutin,quercetin and luteolin were in the range of 68.75~179.41,48.52~232.35,20.45~32.47 and 0.34~1.10 mg/kg.This research would provide a methodology for quality control of active components in sunflower calathide-based products.

关 键 词：葵花盘粉 高效液相色谱 东莨菪素 芦丁 槲皮素 木樨草素 

分 类 号：O65[理学—分析化学]