Pixel-excitation-gating enhanced nonlinear imaging with a GHz femtosecond pulse laser  

作　　者：Wenlong Wang Junpeng Wen Yuke Sheng Wei Lin Chiyi Wei Cihang Kong Dongdan Chen Xiaoming Wei Zhongmin Yang 王文龙;温俊鹏;盛宇柯;林巍;韦池一;孔慈航;陈东丹;韦小明;杨中民(School of Physics and Optoelectronics,South China University of Technology,Guangzhou 510640,China;Institute for Translational Brain Research,Fudan University,Shanghai 200032,China;School of Materials Science and Engineering,Guangdong Engineering Technology Research and Development Center of Special Optical Fiber Materials and Devices,Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques,South China University of Technology,Guangzhou 510641,China;Research Institute of Future Technology,South China Normal University,Guangzhou 510006,China)

机构地区：[1]School of Physics and Optoelectronics,South China University of Technology,Guangzhou 510640,China [2]Institute for Translational Brain Research,Fudan University,Shanghai 200032,China [3]School of Materials Science and Engineering,Guangdong Engineering Technology Research and Development Center of Special Optical Fiber Materials and Devices,Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques,South China University of Technology,Guangzhou 510641,China [4]Research Institute of Future Technology,South China Normal University,Guangzhou 510006,China

出　　处：《Chinese Optics Letters》2024年第12期33-37,共5页中国光学快报(英文版)

基　　金：supported by the Key-Area Research and Development Program of Guangdong Province(No.2023B0909010002);the National Natural Science Foundation of China(Nos.62375087 and 12374304);the NSFC Development of National Major Scientific Research Instrument(No.61927816);the Introduced Innovative Team Project of Guangdong Pearl River Talents Program(No.2021ZT09Z109);the Natural Science Foundation of Guangdong Province(No.2021B1515020074)。

摘　　要：In this Letter,we propose a pixel-excitation-gating technology for high-sensitivity nonlinear imaging excited by femtosecond pulses at a GHz repetition rate,which can enhance the signal quality of nonlinear imaging.To validate this technology,we apply it to a customized two-photon excitation(TPE)fluorescence microscope that uses a GHz femtosecond pulse fiber laser as the excitation source.Compared with the excitation using a continuous GHz pulse train,up to~4-fold enhancement of fluorescence signal can be obtained when the excitation pulse train is gated with a 25%duty cycle of the pixel dwell time,under constant average power.It is anticipated that pixel-level-excitation gating can be an add-on solution for fluorescence signal enhancement in nonlinear imaging systems that are excited by GHz femtosecond pulses.Moreover,the pixel-level excitation control would allow more flexible excitation.

关 键 词：multiphoton fluorescence microscopy GHz femtosecond pulse laser signal enhancement pixel-excitation gating 

分 类 号：TN2[电子电信—物理电子学]