营口市宠物源大肠杆菌耐药性与ESBLs、PMQR耐药基因检测  

Detection of antibiotic resistance and ESBLs,PMQR resistance genes in Escherichia coli isolates from pets in Yingkou city

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作  者:韩彦飞 苗中秋[1] 张利[1] 姜仁礼 刘琪 李美琦 刘正伟[1] HAN Yanfei;MIAO Zhongqiu;ZHANG Li;JIANG Renli;LIU Qi;LI Meiqi;LIU Zhengwei(Liaoning Agricultural College,Yingkou 115009,China)

机构地区:[1]辽宁农业职业技术学院,辽宁营口115009

出  处:《畜牧与兽医》2025年第3期38-46,共9页Animal Husbandry & Veterinary Medicine

基  金:辽宁农业职业技术学院校级课题(Lnz202212)。

摘  要:旨在探究宠物源大肠杆菌对抗菌药物的耐药情况及携带的质粒介导喹诺酮(PMQR)和超光谱β-内酰胺酶(ESBLs)耐药基因的检测。采集营口市307份宠物犬、猫肛拭子样品,分离获得282株大肠杆菌,通过K-B药敏纸片法对分离菌株进行药敏试验;在头孢他啶和头孢噻肟药敏纸片中加入克拉维酸来确定分离菌株是否为ESBLs表型耐药菌株;应用PCR测定大肠杆菌菌株携带的ESBLs和PMQR耐药基因型。结果:宠物犬源、猫源大肠杆菌对β-内酰胺类药物耐药率分别为21.15%~60.00%和22.22%~37.50%;对喹诺酮类药物耐药率分别为25.85%~52.73%和43.75%~57.50%;对氨基糖苷类药物的耐药率分别为7.69%~69.09%和16.67%~57.50%;对四环素类药物的耐药率分别为5.77%~39.13%和18.75%~37.50%;对多肽类药物的耐药率分别为6.52%~20.91%和12.50%~27.78%;对磺胺类药物的耐药率分别为17.31%~29.09%和22.22%~33.78%;经测定,148株大肠杆菌检测为多重耐药菌株,三重耐药菌株检出率最多,共有56株大肠杆菌为产ESBLs表型;在产ESBLs表型大肠杆菌中,blaCTX-M耐药基因检出率最高为28.57%(16/56),其次为blaTEM耐药基因,检出率为17.86%(10/56);共有74株大肠杆菌携带PMQR耐药基因,其中qnrS耐药基因检出率最高为44.59%(33/74),其次为aac(6′)-Ib-cr,检出率为18.92%(14/74)。综上,营口地区宠物犬源、猫源大肠杆菌耐药较为严重,携带多种耐药基因,菌株携带的ESBLs耐药基因以blaCTX-M为主;携带的PMQR耐药基因以qnrS为主。The aim of this experiment was to investigate the resistance of pet-derived Escherichia coli to antibiotics and the carrying ESBLs and PMQR resistance genotypes.Anal swab samples were collected from 307 pet dogs and cats in city of Yingkou.Two hundred and eightytwo strains of Escherichia coli were isolated and tested for drug sensitivity using the K-B drug sensitivity paper method.To determine whether the isolated strain was ESBLs phenotype resistant,clavulanic acid was added to the ceftazidime and cefotaxime sensitive paper slides.Finally,PCR was employed to determine ESBLs and PMQR resistance genotypes carried by the Escherichia coli strains.The results showed that Escherichia coli from the pet dogs and cats had an impact on the resistance rates ofβ-lactams drugs ranging from 21.15%to 60.00%and from 22.22%to 37.50%,respectively.The resistance rates to quinolone drugs ranged from 25.85%to 52.73%and from 43.75%to 57.50%,respectively.The resistance rates to aminoglycoside drugs were 7.69%-69.09%and 16.67%-57.50%,respectively.The resistance rates to tetracycline drugs ranged from 5.77%to 39.13%and from 18.75%to 37.50%,respectively.The resistance rates to peptide drugs were 6.52%-2.91%and 12.50%-27.78%,respectively.And the resistance rates to sulfonamide drugs ranged from 17.31%to 29.09%and from 22.22%to 33.78%,respectively.After testing,148 strains of Escherichia coli were detected as multidrug-resistant strains,with the highest detection rate of triple resistant strains.There were 56 strains of Escherichia coli that produced ESBLs phenotype.Among the ESBLs phenotype Escherichia coli isolates,the detection rate of blaCTX-M resistance gene was the highest,at 28.57%(16/56),followed by the blaTEM resistance gene with a detection rate of 17.86%(10/56).There were a total of 74 Escherichia coli strains carrying PMQR resistance genes,with the highest detection rate of qnrS resistance gene being 44.59%(33/74),followed by aac(6')-Ib-cr with detection rate of 18.92%(14/74).In summary,the E.coli strains from pet dogs and cat

关 键 词:宠物 大肠杆菌 耐药性 耐药基因 

分 类 号:S855[农业科学—临床兽医学]

 

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