不同产地苏合香超高效液相色谱特征图谱及多指标成分含量测定  

UPLC characteristic chromatograms and determination of multiple indicator constituents in different origins of Styrax

作　　者：郭晓晗 丁一明 张宇 杨建波[1] 康帅[1] 荆文光 程显隆[1] 魏锋[1] GUO Xiao-han;DING Yi-ming;ZHANG Yu;YANG Jian-bo;KANG Shuai;JING Wen-guang;CHENG Xian-long;WEI Feng(National Institutes for Food and Drug Control,Beijing 102629,China;School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102629,China;Shenyang Pharmaceutical University,Faculty of Functional Food and Wine,Shenyang 117004,China)

机构地区：[1]中国食品药品检定研究院,北京102629 [2]北京中医药大学中药学院,北京102629 [3]沈阳药科大学功能食品与葡萄酒学院,沈阳117004

出　　处：《药物分析杂志》2025年第1期72-80,共9页Chinese Journal of Pharmaceutical Analysis

基　　金：中国食品药品检定研究院中青年发展研究基金项目(2023A2);国家药品监督管理局药品监管科学体系建设重点项目(RS2024Z006)。

摘　　要：目的:建立苏合香药材的超高效液相色谱(UPLC)特征图谱,并测定苏合香中3个成分(肉桂酸、肉桂酸肉桂酯、肉桂酸-3-苯基丙酯)的含量,旨在研究不同产地、不同形态市售苏合香的质量,为进口药材苏合香的质量控制提供参考。方法:采用UPLC法,使用Acquity UPLC BEH C_(18)(100 mm×2.1 mm,1.7μm)色谱柱,以乙腈-1%乙酸溶液为流动相,梯度洗脱,体积流量0.3 mL·min^(-1),检测波长277 nm,柱温35℃。建立苏合香药材特征图谱,并测定不同来源苏合香药材的3个主要成分的含量,采用ChemPattern软件进行聚类分析(HCA)、主成分分析(PCA)等化学计量方法对苏合香进行质量评价。结果:12批苏合香药材特征图谱共确定10个共有峰,指认了4个特征峰;3个成分(肉桂酸、肉桂酸肉桂酯、肉桂酸-3-苯基丙酯)在各自范围内线性关系良好(r≥0.999);平均回收率(n=6)分别为101.8%(RSD=1.3%)、105.8%(RSD=1.2%)、99.2%(RSD=1.8%)。12批苏合香中3个成分含量的分别为2.74%~3.69%、28.21%~30.63%、16.89%~20.98%。结论:该方法简便易行,准确度高,重复性好,可为苏合香药材质量标准提供整体质量控制依据;结合产地调研信息,对苏合香基原的扩充以及不同国家苏合香标准的协调、提高具有重要的参考意义。Objective:To establish the ultra performance liquid chromatography(UPLC)characterization profile of Styrax,and to determine the contents of three components(cinnamic acid,cinnamyl cinnamate,3-phenylpropyl cinnamate)in Styrax,the aim was to study the quality of commercially available sulforaphane of different origins and forms,and to provide a reference for the quality control of imported medicinal herbs Styrax.Methods:The UPLC method was performed on an Acquity UPLC BEH C_(18)(100 mm×2.1 mm,1.7μm)column with acetonitrile-1%acetic acid solution as the mobile phase in a gradient elution.The volume flow rate was 0.3 mL·min^(-1).The detection wavelength was 277 nm.and the temperature of the column was 35℃.Characteristic chromatograms of styrax were established,and the contents of three major components of Styrax from different sources were determined and the data were statistically analyzed by hierarchical cluster analysis(HCA)and principal component analysis(PCA)with ChemPattern software.Results:The results showed that the UPLC characteristic chromatograms of Styrax was established with 10 common peaks and 4 identified components.The linear relationship of 3 components in their respective ranges was good(r≥0.999),and the average recoveries(n=6)were 101.8%(RSD=1.3%),105.8%(RSD=1.2%),99.2%(RSD=1.8%),respectively.The content of 3 components were 2.74%-3.69%,28.21%-30.63%,16.89%-20.98%,respectively.Conclusion:The method is simple,accurate and reproducible,and can provide an overall quality control basis for the quality standard of Styrax.Combined with the information of origin research,it has important reference significance for the expansion of the origin of Styrax,as well as the harmonization and improvement of the standards of Styrax in different countries.

关 键 词：苏合香 超高效液相色谱 特征图谱 肉桂酸 肉桂酸肉桂酯 肉桂酸-3-苯基丙酯 含量测定 

分 类 号：R917[医药卫生—药物分析学]