CRISPR-Cas9基因编辑技术的应用与展望  

Application and Prospect of CRISPR-Cas9

作　　者：刘婧 郑积敏[1] LIU Jing;ZHENG Ji-Min(College of Chemistry,Beijing Normal University,Beijing 100875,China)

机构地区：[1]北京师范大学化学学院,北京100875

出　　处：《化学教育(中英文)》2025年第4期1-8,共8页Chinese Journal of Chemical Education

摘　　要：基因编辑技术是一系列随时间逐步发展演进的技术,先后经历了锌指核酸酶技术、转录激活剂样效应核酸酶技术以及簇状规则间隔短回文重复序列(CRISPR)与其相关蛋白(Cas9)技术。介绍了这些技术的工作原理,探讨了它们各自的优点与不足,重点阐述了CRISPR-Cas9技术在农作物水稻以及医学疾病癌症研究中的应用实例。最后,介绍了人类对基因编辑技术所引发的伦理和社会问题的深切关注。Gene editing techniques were developed chronologically,which include zinc finger nuclease,transcription activator-like effector nuclease and clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas 9).This paper introduces the working principles of these technologies,explores their respective advantages and disadvantages,and illustrates the application examples of CRISPR-Cas9in rice as well as in medical diseases cancer.Finally,the article presents the deep concerns of humans over the ethical and social issues arising from gene editing technology.

关 键 词：基因编辑 CRISPR-Cas9系统 作物改良 疾病治疗 伦理问题 

分 类 号：Q78[生物学—分子生物学]