健脾愈疡汤对溃疡性结肠炎模型小鼠肠黏膜屏障修复作用的研究  

The Effect of Jianpi Yuyang Decoction(健脾愈疡汤)on the Repair of Intestinal Mucosal Barrier in Mice with Ulcerative Colitis

作　　者：梁巧俐 陈柏辉 陈映西 潘娜 黄晓燕[2] LIANG Qiaoli;CHEN Baihui;CHEN Yingxi;PAN Na;HUANG Xiaoyan(Guangxi University of Traditional Chinese Medicine,Nanning Guangxi 530001,China;The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning Guangxi 530023,China)

机构地区：[1]广西中医药大学,广西南宁530001 [2]广西中医药大学第一附属医院,广西南宁530023

出　　处：《中医药导报》2025年第2期24-29,共6页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广西岐黄学者培养项目(2022015-003-01);“四象脾土和五脏”脾胃治未病创新团队(2021B001);广西科技厅自然科学基金面上项目(2020GXNSFAA238009)。

摘　　要：目的:探讨健脾愈疡汤对溃疡性结肠炎(UC)模型小鼠肠黏膜的修复作用。方法:43只SPF级雄性KM小鼠适应性喂养7 d后,随机选取10只作为正常组,其余为造模组。造模组小鼠自由饮用3%葡聚糖硫酸钠溶液,连续14 d,复制UC小鼠模型。造模完成后,随机选取3只评定模型。模型建立后,将30只小鼠随机分为模型组、西药组、中药组,每组10只。西药组予美沙拉秦肠溶片灌胃,中药组予健脾愈疡汤灌胃,正常组和模型组予等剂量的蒸馏水灌胃,连续14 d。观察小鼠一般情况、疾病活动指数(DAI)评分、组织病理学评分及肠黏膜通透性。采用Western blotting检测结肠组织中ZO-1蛋白、Tricellulin蛋白表达。结果:与正常组比较,模型组小鼠部分出现毛躁、懒动、黏液血便症状,DAI评分、肠黏膜通透性及组织学评分均显著升高(P<0.05),且ZO-1、Tricellulin蛋白相对表达量均显著下降(P<0.05)。与模型组比较,西药组和中药组小鼠DAI评分、肠黏膜通透性、组织学评分均显著下降(P<0.05),西药组结肠ZO-1、Tricellulin蛋白相对表达量均显著升高(P<0.05),中药组结肠ZO-1蛋白相对表达量升高(P<0.05)、Tricellulin蛋白相对表达量未见明显升高。结论:健脾愈疡汤对于UC小鼠肠黏膜具有一定的修复作用。Objective:To investigate the repair effect of Jianpi Yuyang decoction on the intestinal mucosa of ulcerative colitis(UC)model mice.Methods:After 7 days of adaptive feeding,10 of the 43 SPF male KM mice were randomly selected as the normal group,and the rest were made of modules.The mice were copied with 3%dextran sodium sulfate solution for 14 consecutive days,to establish UC mouse model.After the modeling was completed,3 evaluation models were randomly selected.After the model was established,30 mice were randomly divided into model group,Western medicine group,and Chinese medicine group,with 10 mice in each group.The The mice were given mesalazine enteric-coated tablets by gavage in Western medicine group,while Jianpi Yuyang decoction in Chinese medicine group.And the normal group and model group were given the same dose of distilled water by gavage for 14 consecutive days.The general condition,disease activity index(DAI)score,histopathological score and intestinal mucosal permeability of the mice were observed.Western blotting was used to detect the protein expression of ZO-1 and Tricellulin in colon tissues.Results:Compared with the normal group,some mice showed symptoms of frizziness,laziness,mucus and blood in model group.The DAI score,intestinal mucosal permeability and histological scores were significantly increased in model group(P<0.05),while the protein expressions of ZO-1 and Tricellulin were significantly decreased(P<0.05).Compared with the model group,the DAI score,intestinal mucosal permeability and histology scores were significantly decreased in Western medicine group and Chinese medicine group(P<0.05).The contents of ZO-1 and Tricellulin protein were significantly increased in Western medicine group(P<0.05),and the expression of ZO-1 protein was increased in Chinese medicine group(P<0.05),while the Tricellulin protein expression were not significantly increased in Chinese medicine group.Conclusion:Jianpi Yuyang decoction shows a certain repair effect on the intestinal mucosa of UC mice.

关 键 词：溃疡性结肠炎 健脾愈疡汤 肠黏膜屏障 紧密连接蛋白 小鼠 

分 类 号：R285.5[医药卫生—中药学]