人参煮散饮片与传统饮片中皂苷类成分的UHPLC-Q-Exactive Orbitrap MS比较分析  

Comparative Analysis of Ginsenosides in Renshen(Ginseng)Boiling Powder and Renshen(Ginseng)Traditional Decoction Pieces Based on UHPLC-Q-Exactive Orbitrap MS

作　　者：赵雪 张中磊 梁可欣 邓含秀 黄书腾 张如月 王少平 李宁 张加余[1,2] ZHAO Xue;ZHANG Zhonglei;LIANG Kexin;DENG Hanxiu;HUANG Shuteng;ZHANG Ruyue;WANG Shaoping;LI Ning;ZHANG Jiayu(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Ji'nan Shandong 250300,China;College of Traditional Chinese Medicine,Binzhou Medical University,Yantai Shandong 264003,China;College of Pharmacy,Binzhou Medical University,Yantai Shandong 264003,China;KMHD GeneTech Co.Ltd.,Shenzhen Guangdong 518128,China)

机构地区：[1]山东中医药大学药学院,山东济南250300 [2]滨州医学院中医学院,山东烟台264003 [3]滨州医学院药学院,山东烟台264003 [4]康美华大基因技术有限公司,广东深圳518128

出　　处：《中医药导报》2025年第2期47-56,共10页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：山东省泰山学者青年专家项目(tsqn202103110);山东省青创人才引育计划——中药复杂体系作用模式解析研究创新团队(10073004)。

摘　　要：目的:分析人参煮散饮片与传统饮片在煎煮过程中的化学成分相对含量变化,为煮散的研究及临床应用提供参考。方法:取3批人参传统饮片加工制成煮散饮片,应用UHPLC-Q-Exactive Orbitrap高分辨液质联用技术,筛选并鉴定煎煮过程中变化显著的皂苷类化学成分。结果:共鉴定了70种皂苷成分,峰面积差异率(ω)≥50%的化合物共4种,50%>ω≥10%的化合物共26种,10%>ω≥0%的化合物共28种。加权结果显示,人参煮散饮片1.00 g相当于传统饮片1.39 g。结论:人参煮散饮片与传统饮片药效基本一致,但煮散饮片具有较高的有效成分溶出率,可以降低成本。Objective:To study the relative content changes of chemical components in the decoction process of renshen(ginseng)boiling powder and renshen(ginseng)traditional decoction pieces to provide a reference for the study and clinical application of boiling powder.Methods:A total of 3 batches of renshen(ginseng)traditional decoction pieces were processed into boiling powder,and UHPLC-Q-Exactive Orbitrap high-resolution liquid mass spectrometry technique was used to screen and identify the saponins with significant changes in the decoction process.Results:A total of 70 ginsenosides were identified,including 4 compounds with peak area difference rate(ω)≥50%,26 compounds with 50%>ω≥10%,and 28 compounds with 10%>ω≥0%.The weighted results showed that 1.00 g renshen(ginseng)boiling powder was equivalent to 1.39 g traditional renshen(ginseng)pieces.Conclusion:The efficacy of renshen(ginseng)boiling powder is basically the same as that of traditional decoction pieces,but the boiling powder have higher dissolution rate of active ingredients,which can reduce the cost.

关 键 词：人参 人参煮散 人参皂苷 对比分析 

分 类 号：R284.1[医药卫生—中药学]