古方上党参膏提取工艺及免疫调节药效评价  

Extraction technology of the ancient formula Shangdangshen Ointment and the evaluation of its immunomodulatory efficacy

作　　者：姬惠鑫 高敏 温嘉琪[1] 王利珍 白云娥 高建平[1,2,3] JI Huixin;GAO Min;WEN Jiaqi;WANG Lizhen;BAI Yun'e;GAO Jianping(School of Pharmacy,Shanxi Medical University,Shanxi Taiyuan 030001,China;Medicinal Basic Research Innovation Center of Chronic Kidney Disease,Ministry of Education,Shanxi Medical University,Shanxi Taiyuan 030001,China;Shanxi Provincial Key Laboratory of Drug Synthesis and Novel Pharmaceutical Preparation Technology,Shanxi Medical University,Shanxi Taiyuan 030001,China;Forestry Administration Station of Kelan County Forestry Bureau,Shanxi Province,Shanxi Xinzhou 036300,China)

机构地区：[1]山西医科大学药学院,山西太原030001 [2]教育部慢性肾脏病医药基础研究创新中心,山西太原030001 [3]山西省药物合成和制剂新技术重点实验室,山西太原030001 [4]山西省岢岚县林业局林政站,山西忻州036300

出　　处：《中国医院药学杂志》2025年第2期121-127,共7页Chinese Journal of Hospital Pharmacy

基　　金：国家重点研发计划课题(编号:2018YFC1706304);山西省高等学校科技创新计划项目(编号:2021L235)。

摘　　要：目的:优选古方上党参膏(Shangdangshen Ointment,SO)最佳提取工艺,并初步评价其对小鼠免疫功能的影响。方法:以提取次数、加水量、提取时间为自变量,以党参炔苷含量、出膏率的综合评分为提取工艺的评价指标,采用层次分析法(AHP)-指标权重确定法(CRITIC)复合熵权确定评价指标的权重系数,基于Box-Behnken响应面法设计实验,优化上党参膏的最佳提取工艺。腹腔注射环磷酰胺建立免疫抑制小鼠模型,采用香菇多糖和上党参膏灌胃进行治疗30 d,检测小鼠体质量、免疫器官脏器指数、血清免疫因子(IL-2、IL-4)水平,HE染色观察小鼠肠道病理表现。结果:上党参膏的最佳提取工艺:提取3次,分别加10倍、8倍、6倍量水,第1次提取1 h,第2、3次提取0.5 h。与空白组比较,模型组小鼠体质量、胸腺指数显著下降(P<0.01),脾脏指数、血清免疫因子水平显著升高(P<0.01),小肠组织结构损伤严重;上党参膏治疗组小鼠体质量、胸腺指数显著升高(P<0.01),脾脏指数、血清免疫因子水平显著降低(P<0.05),小肠组织结构损伤明显减轻。结论:基于BoxBehnken响应面法优选的上党参膏提取工艺可操作性强、提取率高、节能、重现性好;上党参膏可明显改善环磷酰胺致免疫功能低下小鼠的免疫功能。OBJECTIVE To optimize the extraction technology of the ancient formula Shangdangshen Ointment(SO),and to evaluate its effect on mouse immune function.METHODS Independent variables included extraction frequency,the amount of water added,and extraction time,and evaluation parameters included the content of lobetyolin,and the comprehensive score of the ointment yield.The weight coefficient of each evaluation index was determined by the composite entropy weight of analytic hierarchy process(AHP)-index weight determination method(CRITIC).Box-Behnken response surface method was to identify the optimal extraction technology of SO.An immunosuppressive mouse model was established by intraperitoneal injection of cyclophosphamide,followed by oral gavage of Lentinan and SO for 30 days.The body weight,organ index of immune organs,and serum immune factors(interleukin IL-2,IL-4) were detected,and the intestinal pathology of mice was observed by hematoxylin and eosin(H&E) staining.RESULTS The optimal extraction process for SO was three extractions(1 h,0.5 h and 0.5 h,respectively) by adding 10-fold,8-fold and 6-fold,respectively.Compared with the blank group,mouse in the model group had significantly decreased body weight and thymus index,increased spleen index and serum immune factors(all P<0.01),and seriously damaged structure of small intestine.Model mice treated with SO had significantly increased body weight and thymus index(P<0.01),and decreased spleen index and serum immune factors(P<0.05).The impaired structure of small intestinal tissue was significantly alleviated.CONCLUSION Based on the Box-Behnken response surface methodology,the optimized extraction process of SO has the advantages of strong operability,high extraction rate,energy saving and good reproducibility.SO can significantly improve the immune function of immunocompromised mice induced by cyclophosphamide.

关 键 词：上党参膏 提取工艺 Box-Behnken响应面法 党参炔苷 免疫调节 

分 类 号：R284.2[医药卫生—中药学] R943[医药卫生—中医学]