超声波辅助低共熔溶剂提取淫羊藿总黄酮的工艺优化及其降糖活性研究  

Optimization of extracting total flavonoids from Epimedium brevicornu by ultrasonic-assisted deep eutectic solvent and their hypoglycemic activity

作　　者：肖咪 罗蓉[1] 赵红玉 苏彦奇 李丹[2] 蔡旭 余涛[3] XIAO Mi;LUO Rong;ZHAO Hongyu;SU Yanqi;LI Dan;CAI Xu;YU Tao(Pharmaceutical Preparation Section,Union Jiangbei Hospital,Huazhong University of Science and Technology,Wuhan Caidian People's Hospital,Hubei Wuhan 430100,China;Department of Pharmacy,Renmin Hospital of Wuhan University,Hubei Wuhan 430060,China;Department of Oncology of Integrated Chinese and Western Medicine,the Central Hospital of Wuhan,Tongji Medical College,Huazhong University of Science and Techonology,Hubei Wuhan 430014,China)

机构地区：[1]华中科技大学协和江北医院/武汉蔡甸区人民医院药学部,湖北武汉430100 [2]武汉大学人民医院药学部,湖北武汉430060 [3]华中科技大学同济医学院附属武汉中心医院中西医结合肿瘤科,湖北武汉430014

出　　处：《中国医院药学杂志》2025年第2期135-140,共6页Chinese Journal of Hospital Pharmacy

基　　金：华中科技大学协和江北医院自由创新预研基金(编号:XHJBKXYJJJ-XYCX-05);武汉市卫健委中医药科研面上项目(编号:WZ22A07)

摘　　要：目的:考察低共熔溶剂提取淫羊藿总黄酮的效果并优化其提取工艺。方法:以淫羊藿总黄酮得率为指标,制备10种低共熔溶剂,从中筛选提取率最佳溶剂。在单因素试验(液料比、超声时间、提取温度)基础上,利用响应面法优化低共熔溶剂提取淫羊藿总黄酮的液料比(mL·g^(-1))、提取温度(℃)及超声时间(min),获得最佳提取工艺。同时,通过降糖活性,验证最佳提取工艺。结果:含质量分数为40%水的1,3-丙二醇/氯化胆碱(摩尔比2∶1)低共熔溶剂作为提取淫羊藿总黄酮的提取溶剂,其最佳提取工艺为超声时间27 min、液料比44 mL·g^(–1),提取温度为72℃。在此条件下,淫羊藿总黄酮提取率为8.26%,降糖活性IC50为0.31 mg·mL^(-1),均显著高于甲醇提取组。结论:氯化胆碱/1,3-丙二醇是提取淫羊藿总黄酮的良好溶剂,可有效提升淫羊藿总黄酮提取率,为淫羊藿的开发利用提供科学依据。OBJECTIVE To investigate the extraction effect of total flavonoids from Epimedium brevicornu(E.brevicornu) by using ultrasonic-assisted deep eutectic solvents(DESs),and to optimize the extraction parameters.METHODS Using the yield of total flavonoids of E.brevicornu as an indicator,ten DESs were prepared for extracting total flavonoids of E.brevicornu,and that with the highest yield was identified.Then,the response surface methodology was employed to optimize the extraction process based on the univariate experiments [extraction temperature (℃ ),liquid-solid ratio(mL·g^(–1)) and ultrasonic time(min)],and obtain the optimal process parameters.Meanwhile,the optimal extraction process was validated by in-vitro hypoglycemic activity evaluation.RESULT 1,3-propanediol/choline chloride(molar ratio 2∶1) containing 40 wt% water was chosen as the optimal extraction solvent,and the optimum extraction parameters were as follow:ultrasonic time(27 min),liquid-solid ratio(44 mL·g^(–1)) and extraction temperature(72 ℃).Under the above extraction condition,the yield of total flavonoids was 8.26%.Total flavonoids showed an IC50 of 0.31 mg·mL^(–1) on the hypoglycemic activity,which was superior to the alcohol extraction method.CONCLUSION 1,3-propanediol/choline chloride is a good solvent for extracting total flavonoids from E.brevicornu,which can effectively improve the yield of total flavonoids,and provide the scientific evidence for the resource development and utilization of E.brevicornu.

关 键 词：低共熔溶剂 淫羊藿 总黄酮 

分 类 号：R932[医药卫生—生药学]