一种基于三苯胺的脂滴靶向荧光探针的合成及性质研究  

On Synthesis and Properties of a Lipid Droplets Targeted Fluorescent Probe Based on Triphenylamine

作　　者：邓奕 王志宇 汪捷 吴运军 王慧 胡磊 DENG Yi;WANG Zhiyu;WANG Jie;WU Yunjun;WANG Hui;HU Lei(Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院药学院,安徽芜湖241002

出　　处：《滁州职业技术学院学报》2024年第4期37-40,共4页Journal of Chuzhou Polytechnic

基　　金：安徽省自然科学基金(1908085QB50);安徽省大学生创新创业教育训练计划项目(S202310368086,S202310368089)。

摘　　要：以三苯胺和1,3-茚满二酮为基本构筑单元,通过Knoevenagel缩合反应设计并合成了一种D-π-A构型的化合物,运用核磁共振氢谱和质谱对化合物的结构进行了表征。借助紫外可见吸收光谱、荧光光谱和理论计算考察了化合物的光物理性质,发现其紫外吸收峰基本不受溶剂极性的影响(除水外),荧光光谱却显示出较强的溶剂化效应。MTT实验和激光共聚焦细胞成像实验结果表明,化合物的细胞毒性较低,可靶向于活细胞中的脂滴部位。A D-π-A structural compound has been designed and synthesized through Knoevenagel reaction using triphenylamine and indane-1,3-dione as the basic building blocks.The structure of the compound is characterized by 1 H NMR and LC-MS.The photophysical properties of this compound have been investigated with UV-vis absorption spectra,fluorescence spectra and theoretical calculations.It is found that the absorption peak of this compound is not affected by the polarity of solvents(except water),but the fluorescence spectra show a strong solvation effect.The results of MTT experiment and cell imaging experiment demonstrate that the compound has low cytotoxicity and could target the lipid droplets in living cells.

关 键 词：三苯胺 1 3-茚满二酮 荧光性质 脂滴 细胞成像 

分 类 号：O625.6[理学—有机化学]