草鱼抗原加工相关转运蛋白TAP的系统进化及其响应细菌和病毒感染的转录表达分析  

Phylogeny of grass carp TAP genes and their expression kinetics in response to bacterial and viral infection

作　　者：雷文晶 徐兢 贾钊 柳轶帆 郭旭 王俊亚 邹钧 LEI Wenjing;XU Jing;JIA Zhao;LIU Yifan;GUO Xu;WANG Junya;ZOU Jun(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306

出　　处：《上海海洋大学学报》2025年第1期94-110,共17页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(32030112)。

摘　　要：克隆了草鱼(Ctenopharyngodon idella)TAP1、TAP2a、TAP2t基因、分析了其在系统进化中的地位,发现草鱼TAP基因结构非常保守,与鲤科鱼类亲缘关系最近。通过对TAP蛋白的结构预测发现草鱼TAP蛋白含有多个跨膜区,并具有ATP结合盒式蛋白(ATP-binding cassette transporter, ABC)超家族的典型结构特征。草鱼TAP基因在脾脏和头肾组织中表达量较高;感染嗜水气单胞菌(Aeromonus hydrophila)72 h后,TAP1、TAP2a、TAP2t表达显著上调。植物血凝素(Phytohemagglutinin, PHA)和聚肌苷酸-聚胞苷酸(Polyinosinicpolycytidylic acid)均可诱导草鱼TAP1、TAP2a、TAP2t的表达。此外,分析了细胞因子对TAP基因表达的影响,结果显示,伽马干扰素(IFN-γ)和白介素1beta(IL-1β)上调TAP1和TAP2t的表达。EPC细胞在感染鲤春病毒血症病毒(Spring viraemia of carp virus, SVCV)12、24和36 h后TAP1的表达量显著升高,TAP2t表达在12 h得到上调,提示TAP1和TAP2t参与宿主应答SVCV的免疫反应。研究结果为阐明鱼类MHC I的抗原呈递机制奠定基础。In this study,we cloned the TAP1,TAP2a,and TAP2t genes from grass carp(Ctenopharyngodon idella),and analyzed their phylogeny.It was found that grass carp TAP genes are conserved.Structural prediction of TAP proteins revealed that grass carp TAP proteins possess multiple transmembrane regions and have typical structural features of the ATP-binding cassette transporter(ABC)superfamily.Grass carp TAP1,TAP2a,and TAP2t genes were highly expressed in the spleen and kidney,and significantly upregulated at 72h after infection with Aeromonas hydrophila.In the primary head kidney leukocytes,stimulation with phytohemagglutinin(PHA)and polyinosinic-polycytidylic acid[poly(I:C)]upregulated all TAP genes,and that with interferon gamma(IFN-γ)and interleukin 1 beta(IL-1β)induced the expression of TAP1 and TAP2t but not TAP2a.In the EPC cells,infection with Spring viraemia of carp virus(SVCV)elevated the expression levels of TAP1 at 12 h,24 h,and 36 h and TAP2t at 12 h,suggesting they are involved in immune response to SVCV infection.The findings provide valuable information for further investigation on MHC I antigen presentation pathway in fish.

关 键 词：抗原加工相关转运蛋白TAP MHCⅠ 草鱼 转录表达分析 细菌 病毒 

分 类 号：S917[农业科学—水产科学]