打斗失败经历通过血清素通路对中华绒螯蟹焦虑样行为的影响  

The effects of the loser experience induces anxiety-like behavior through the serotonin pathway on Chinese mitten crab(Eriocheir sinensis)

作　　者：张颖[1,2] 雷金沄 成永旭[1,2] 杨筱珍[1,2] ZHANG Ying;LEI Jinyun;CHENG Yongxu;YANG Xiaozhen(Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学上海水产养殖工程技术研究中心,上海201306 [2]上海海洋大学水产科学国家级实验教学示范中心,上海201306

出　　处：《上海海洋大学学报》2025年第1期135-145,共11页Journal of Shanghai Ocean University

基　　金：国家重点研发计划(2023YFD2401902);国家自然科学基金(41876190);财政部和农业农村部国家现代农业产业技术体系(CARS-48);上海市科委水产养殖工程研究平台项目(19DZ2284300);黄河三角洲行业领军人才项目(DYRC20190210)。

摘　　要：在中华绒螯蟹(Eriocheir sinensis)养殖过程中,普遍存在着打斗现象,为探究打斗失败经历带来的不良影响,观察了经历打斗失败后中华绒螯蟹的焦虑样行为和摄食行为,并检测了血清素(5-HT)水平和血清素7型受体(5-HT7R)基因相对表达水平的变化。随后观察外源性血清素、氟西汀、血清素7型受体激动剂和拮抗剂处理后对中华绒螯蟹焦虑样行为的影响。研究结果显示,经历过打斗失败的中华绒螯蟹会表现出焦虑样行为,并伴随着血浆血清素浓度的升高和胸神经节血清素7型受体基因相对表达水平的下调,且打斗失败后中华绒螯蟹摄食量减少。通过外源性血清素处理促进了中华绒螯蟹的焦虑样行为,而氟西汀处理则减轻了中华绒螯蟹的焦虑样行为。此外,血清素7型受体激动剂(AS19)处理促进了中华绒螯蟹的焦虑样行为,而拮抗剂(SB-269970)处理则减轻了中华绒螯蟹的焦虑样行为。以上结果说明,打斗失败经历会诱导中华绒螯蟹产生焦虑样行为和食欲减退,并且通过血清素途径影响焦虑样行为。本研究揭示了打斗失败经历带来的潜在负面影响,为促进中华绒螯蟹的健康养殖提供理论依据。Agonistic behavior is a common phenomenon in the culture of Eriocheir sinensis.To explore the adverse effects of loser experience,anxiety-like behavior and feeding behavior of E.sinensis after loser experience were observed,and the levels of serotonin(5-HT)and 5-HT7R gene relative expression were detected.Then,the effects of exogenous 5-HT,fluoxetine,and 5-HT7R agonists and antagonists on the anxiety-like behavior of E.sinensis were observed.The results indicated that the E.sinensis that experienced the fight failure showed anxiety-like behavior,which was accompanied by the increase of plasma 5-HT concentration and the decrease of 5-HT7R gene relative expression level in the thoracic ganglion,and the food intake decreased after the loser experience.Exogenous 5-HT treatment promoted anxiety-like behavior,while fluoxetine treatment alleviated anxiety-like behavior.In addition,treatment with 5-HT7R agonist(AS19)promoted anxiety-like behavior,while treatment with 5-HT7R antagonist(SB-269970)alleviated anxiety-like behavior.These results indicate that the loser experience can lead to anxiety-like behavior and affect anxiety-like behavior through the serotonin pathway.This study revealed the potential negative effects ofloser experience and provided theoretical basis for promoting healthy culture of E.sinensis.

关 键 词：中华绒螯蟹 焦虑样行为 血清素 血清素7型受体 

分 类 号：S917[农业科学—水产科学]