可溶性血管细胞黏附分子-1在川崎病患儿中的表达及对血管内皮细胞凋亡的影响  

Expression of Soluble Vascular Cell Adhesion Molecule-1 in Pediatric Kawasaki Disease and Its Effect on Endothelial Cell Apoptosis

作　　者：张爽 马超凡 刘智伟 刘莉莉 王宁 程宇 ZHANG Shuang;MA Chaofan;LIU Zhiwei;LIU Lili;WANG Ning;CHENG Yu(Department of Pediatrics,Tianjin Dongli Hospital,Tianjin 300399,China;a.Department of Plastic and Burn Surgery,Tianjin First Central Hospital,Tianjin 300192,China;Department of Thyroid,Breast and Hernia Surgery,Tianjin First Central Hospital,Tianjin 300192,China)

机构地区：[1]天津市东丽医院儿科,天津300399 [2]天津市第一中心医院烧伤与整形外科,天津300192 [3]天津市第一中心医院甲乳疝外科,天津300192

出　　处：《医学综述》2025年第6期753-760,共8页Medical Recapitulate

基　　金：北京健康促进会中青年医生研究项目(BJHPA-2022-JZHXZHQNYJ-LCH-12)。

摘　　要：目的探讨可溶性血管细胞黏附分子-1(sVCAM-1)在川崎病患儿中的表达及对血管内皮细胞凋亡的影响。方法选择2022年1月至2024年6月天津市东丽医院儿科收治的40例川崎病患儿作为观察组,选取同期体检的40名健康儿童为对照组,对比两组受试者血清sVCAM-1水平,并分析其与白细胞计数(WBC)、C反应蛋白(CRP)水平的相关性。体外培养人冠状动脉内皮细胞(HCAEC),根据sVCAM-1处理浓度不同分为空白组(0μg/L)、低浓度处理组(10μg/L)、高浓度处理组(100μg/L),噻唑蓝法检测3组细胞增殖活力,膜联蛋白V-异硫氰酸荧光素/碘化丙啶双染法检测细胞凋亡情况,Western blot法检测核因子κB(NF-κB)水平,酶联免疫吸附测定法检测B细胞淋巴瘤/白血病-2(Bcl-2)、Bcl-2相关X蛋白(Bax)和胱天蛋白酶3(caspase-3)水平。将高浓度处理组HCAEC分为无NF-κB抑制剂组和NF-κB抑制剂组,分析凋亡相关指标水平变化。结果观察组血清sVCAM-1水平高于对照组[(239±34)ng/L比(69±28)ng/L](P<0.01),sVCAM-1水平与WBC、CRP水平均呈正相关(r=0.443,P<0.001;r=0.720,P<0.001)。不同浓度sVCAM-1处理培养24、48 h,低浓度处理组和高浓度处理组增殖活力显著低于空白组(P<0.01),且高浓度处理组显著低于低浓度处理组[(69.4±3.7)%比(78.7±1.8)%、(25.9±5.7)%比(43.8±7.5)%](P<0.01)。低浓度处理组和高浓度处理组细胞凋亡率、NF-κB表达显著高于空白组(P<0.01),且高浓度处理组显著高于低浓度处理组[(62.6±4.1)%比(29.0±4.3)%、2.42±0.48比1.17±0.12](P<0.01)。低浓度处理组和高浓度处理组Bcl-2表达水平显著低于空白组(P<0.05或P<0.01),且高浓度处理组显著低于低浓度处理组(34±6比76±8)(P<0.01);低浓度处理组和高浓度处理组Bax、caspase-3表达显著高于空白组(P<0.05或P<0.01),且高浓度处理组显著高于低浓度处理组(146±10比66±13、195±20比106±15)(P<0.05或P<0.01)。NF-κB抑制剂组NF-κB、Bax、caspase-3表达以及Objective To investigate the expression of soluble vascular cell adhesion molecule-1(sVCAM-1)in the serum of children with Kawasaki disease and its effect on the apoptosis of vascular endothelial cells.Methods Forty children with Kawasaki disease admitted to the Department of Pediatrics of Tianjin Dongli Hospital from Jan.2022 to Jun.2024 were included as an observation group,and 40 healthy children who underwent physical examination during the same period were included as a control group.The levels of sVCAM-1 in the serum of the two groups were compared,and the correlation with white blood cell count(WBC)and C-reactive protein(CRP)levels was analyzed.Human coronary artery endothelial cells(HCAEC)were cultured in vitro and divided into a blank group(0μg/L),a low concentration treatment group(10μg/L),and a high concentration treatment group(100μg/L)based on the different concentrations of sVCAM-1 treatment.The thiazolyl blue method was used to detect the proliferation activity of the three groups of cells,the annexin V-fluorescein isothiocyanate/propidium iodide double staining method was used to detect apoptosis,the Western blot method was used to detect the expression level of nuclear factor-κB(NF-κB),and the enzyme-linked immunosorbent assay method was used to determine the expression levels of B-cell lymphoma/leukemia-2(Bcl-2),Bcl-2-associated X protein(Bax)and caspase-3.The HCAEC cells in the high concentration treatment group were divided into a non-NF-κB inhibitor group and a NF-κB inhibitor group,and the changes in apoptosis-related indicators were analyzed.Results The serum sVCAM-1 level in the observation group was significantly higher than that in the control group[(239±34)ng/L vs(69±28)ng/L](P<0.01).Its expression level was positively correlated with WBC and CRP levels(r=0.443,P<0.001;r=0.720,P<0.001).After 24 h and 48 h treatment of different concentrations of sVCAM-1,the proliferation activity of low concentration and high concentration groups was significantly lower than that of the blank g

关 键 词：川崎病 可溶性血管细胞黏附分子-1 冠状动脉损伤 内皮细胞 凋亡 

分 类 号：R725.4[医药卫生—儿科]