薯蓣皂苷元对非酒精性脂肪性肝病小鼠脂质代谢的影响  

Impacts of Diosgenin on Lipid Metabolism in Non-alcoholic Fatty Liver Disease Mice

作　　者：梁淑芳 黄国开 聂姝常 程彬彬 刘群 LIANG Shufang;HUANG Guokai;NIE Shuchang;CHENG Binbin;LIU Qun(Changhai Hospital,Shanghai 200433,China;Naval Medical University,Shanghai 200433,China)

机构地区：[1]海军军医大学第一附属医院,上海200433 [2]海军军医大学,上海200433

出　　处：《西部中医药》2025年第2期6-10,共5页Western Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81774077);国家自然科学基金青年基金(82405513);海军军医大学第一附属医院基础研究专项(2023QD21);海军军医大学第一附属医院青年启动基金项目(2019QNA06)。

摘　　要：目的:探讨薯蓣皂苷元对高脂饮食诱导的非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)模型小鼠肝组织脂质沉积与血清甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)水平的影响。方法:将40只雄性ICR小鼠按随机数字表法分为对照组、模型组、薯蓣皂苷元高、低剂量组及非诺贝特组,每组8只。对照组予普通饲料;模型组予45%脂肪供能的高脂饲料;薯蓣皂苷元高、低剂量组分别予薯蓣皂苷元混合饲料,即每千克高脂饲料中分别含薯蓣皂苷元10 g和5 g;非诺贝特组予非诺贝特混合饲料,即每千克高脂饲料中含非诺贝特200 mg,连续干预16周。应用苏木精-伊红染色法(hematoxylin-eosin staining,HE)观察肝组织病理形态学变化;油红O染色法观察肝脏脂肪沉积情况;测定血清TG和TC表达水平。结果:HE染色结果显示:模型组小鼠脂肪变性程度较对照组严重,薯蓣皂苷元高、低剂量组和非诺贝特组肝细胞胞浆内空泡样变减轻。油红O染色结果显示:模型组小鼠肝组织脂肪含量高于对照组,而薯蓣皂苷元高、低剂量组及非诺贝特组肝细胞脂肪含量降低。与对照组比较,模型组小鼠血清TG和TC水平均升高(P<0.01);与模型组比较,薯蓣皂苷元高、低剂量组和非诺贝特组TG和TC水平均降低(P<0.01);与非诺贝特组比较,薯蓣皂苷元高、低剂量组TG和TC表达水平差异无统计学意义(P>0.05)。结论:薯蓣皂苷元能够降低高脂饮食诱导的NAFLD模型小鼠肝细胞脂肪变性程度和脂肪含量及血清TG和TC表达水平。Objective:To survey the influence of diosgenin on the levels of TG and TC,lipid deposition in liver tissue of the mice with non-alcoholic fatty liver disease(NAFLD)induced by high fat diet.Methods:Forty male ICR mice were allocated to the control group,the model group,high and low dosage groups of diosgenin,and fenofibrate group with eight in each group.The control group was fed with common fodder,the model group was given high-fat feed with 45%fat for energy;diosgenin mixed forage,that is,each kilogram of high-fat feed contained 10 g and 5 g of diosgenin respectively,was administered to high and low dosage groups of diosgenin respectively;fenofibrate-mixed fodder,that is,each kilogram of high-fat feed included fenofibrate 200 mg,was given to fenofibrate group,all the groups accepted 16 consecutive weeks of the treatment.HE staining was applied to observe the pathomorphology of liver tissue;oil red O(ORO)staining was utilized to observe the fat deposition in the liver;to detect the expressions of serum TG and TC.Results:HE staining results displayed that the degrees of adipose degeneration of the model group were severer than these of the control group,intracytoplasmic vacuolation was reduced in high and low dosage groups of diosgenin,and fenofibrate group.ORO staining showed that:fat contents of the liver tissue of the model group were higher than these of the control group,while fat contents in hepatic cell were lowered in high and low dosage groups of diosgenin,and fenofibrate group.Compared with the control group,the levels of TG and TC were elevated in the model group(P<0.01);Compared with the model group,the levels of TG and TC were lowered in high and low dosage groups of diosgenin,and fenofibrate group(P<0.01);no statistical difference was found in the levels of TG and TC when high and low dosage groups of diosgenin were compared with fenofibrate group(P>0.05).Conclusion:Diosgenin could reduce adipose degeneration in hepatic cells and fat contents in NAFLD mice models induced by high-fat food,and the expr

关 键 词：脂肪性肝病 非酒精性 脂质代谢 薯蓣皂苷元 小鼠 

分 类 号：R256.49[医药卫生—中医内科学]