丛生鳗草对高温胁迫的分子响应  

Molecular Response of Zostera caespitosa under Thermal Stress

作　　者：文云 童泽录 钟鸣宇 雍玉顺 周悦 马瑞雪 张全胜[1] WEN Yun;TONG Zelu;ZHONG Mingyu;YONG Yushun;ZHOU Yue;MA Ruixue;ZHANG Quansheng(School of Ocean,Yantai University,Yantai 264005,China;Liaocheng Fresh Water Breeding Center,Liaocheng 252000,China)

机构地区：[1]烟台大学海洋学院,山东烟台264005 [2]聊城市淡水养殖试验场,山东聊城252000

出　　处：《烟台大学学报(自然科学与工程版)》2025年第1期30-36,共7页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：国家自然科学基金资助项目(41376154);联合国开发计划署项目(00138351);烟台大学2021年度校地融合发展项目。

摘　　要：从生理、转录两个层面考察了高温胁迫条件下丛生鳗草损伤和防御机制,以认识气候变化背景下海洋升温对海草的影响。结果表明,丛生鳗草在15~30℃的急速升温过程中,快速叶绿素荧光诱导动力学曲线(OJIP)未见明显变化,PSII最大光化学效率(F_(v)/F_(m))维持相对稳定。基因表达分析结果表明,光合作用对热胁迫最敏感,表现在PSII、ATP合酶等基因显著下调;起抗氧化作用的甜菜碱和抗氧化酶POD优先发挥保护功能;非酶抗氧化剂类黄酮基因以及起维持蛋白质结构稳定作用的热休克蛋白Hsp20、Hsp70、Hsp90在亚高温情况下表达;糖酵解途径随温度升高显著表达,作为一种防御机制表达为机体提供能量,从而减缓热损伤。This study investigated the changes in damage and defense mechanisms in Zostera caespitosa under thermal stress at both physiological and transcriptomic levels,aiming to understand the impact of ocean warming in the context of climate change on seagrasses.Results show that during the rapid temperature increase from 15℃to 30℃,no significant alteration was observed in the fast chlorophyll a fluorescence induction curve(OJIP)of Z.caespitosa,while the maximum photochemical efficiency of PSII(F_(v)/F_(m))remains relatively stable.Gene expression results demonstrate the extremely sensitivity of photosynthesis genes to thermal stress,as reflected in the significant downregulation of genes like those of PSII and ATP synthase.Antioxidants such as betaine and the antioxidant enzyme POD take precedence in providing protective functions.Non-enzymatic antioxidants,flavonoid genes,and heat shock proteins(Hsp20,Hsp70,and Hsp90)responsible for maintaining protein structural stability,are expressed under sub-high-temperature conditions.Additionally,as the temperature increases,the glycolysis pathway,which act as a defense mechanism,is significantly up-expressed.

关 键 词：丛生鳗草 高温胁迫 叶绿素荧光 转录组 

分 类 号：Q945.78[生物学—植物学]