小麦植物磺肽激素基因(TaPSK)家族生物信息学及表达模式分析  

Bioinformatics and Expression Pattern Analysis of Phytosulfokine Gene(TaPSK)Family in Triticum aestivum

作　　者：唐凯月 赵晓彤 陈磊[1] 张立培 宋建成[1] 赵吉强[1] TANG Kaiyue;ZHAO Xiaotong;CHEN Lei;ZHANG Lipei;SONG Jiancheng;ZHAO Jiqiang(School of Life Sciences,Yantai University,Yantai 264005,China;Yantai Jien Biotechnology Co.,Ltd.,Yantai 264006,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264005 [2]烟台吉恩生物科技有限公司,山东烟台264006

出　　处：《烟台大学学报(自然科学与工程版)》2025年第1期37-46,共10页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：山东省自然科学基金资助项目(ZR2019BC056);烟台市经济技术开发区科技创新领军团队项目(2017-39)。

摘　　要：为探究TaPSK基因家族成员在根系发育中的作用,本研究利用生物信息学对其进行鉴定分析,通过转录组数据分析TaPSK基因在小麦不同组织的表达模式以及基因所在的染色体位置、基因结构、顺式作用元件等。结果表明:12个TaPSK中有11个属于TaPSK基因家族,TaPSK4属于PSK基因超家族,12个基因分布在A、B、D 3个基因亚族的9条染色体上;RNA-seq数据分析表明,11个TaPSK基因表达具有组织特异性,其中TaPSK3、TaPSK9~11基因在根系不同发育时期表达量均较高;从转录组数据中选取在根系中表达的10个TaPSK基因进行qRT-PCR验证,发现上述4个基因的确在苗期根中有着较高表达水平;顺式作用元件分析发现:TaPSK基因家族成员具有促进根系生长发育、应对胁迫响应及激素调控方面的响应元件。本研究结果可为后续深入研究TaPSK基因根系发育相关的生物学功能提供一定理论基础。To explore the roles of TaPSK gene family members in root development,bioinformatics analysis is used to identify their members.Transcriptome data are used to analyze the expression patterns of TaPSK genes in different tissues of Triticum aestivum as well as chromosomal locations,gene structures,cis acting element predictions.Results as follows:Among the 12 TaPSK genes,11 belong to the TaPSK gene family,of which TaPSK4 belongs to the PSK superfamily,distributing across nine chromosomes of subgenomes A,B and D.Based on the analysis of RNA-seq data,the expression of 11 TaPSK genes is tissue-specific,with TaPSK3,TaPSK90x0E䥺Symbolm@@0x0F11 genes all showing high expression levels at different developmental stages of the root system.10 TaPSK genes expressed in roots are selected from transcriptome data for qRT-PCR verification,and it is found that the above 4 genes are indeed expressed at a higher level in seedling stage roots.Cis acting element analysis identifies that TaPSK gene members play great role in promoting growth and development,responding to stress,and regulating hormones.Results of this study will provide theoretical basis for subsequent in-depth studies on the biological functions related to root development of TaPSK genes.

关 键 词：小麦 根发育 植物磺肽激素 TaPSK基因家族 基因表达 生物信息学分析 

分 类 号：S512.5[农业科学—作物学]