云南红豆杉WRKY基因家族鉴定及表达模式分析  

Identification and Expression Pattern Analysis of WRKY Gene Family in Taxus yunnanensis

作　　者：甘胜美 赵燕波 李洪非 王勇[1] 王刚[1] 黄雄[1] GAN Shengmei;ZHAO Yanbo;LI Hongfei;WANG Yong;WANG Gang;HUANG Xiong(National Forestry and Grassland Southwest Engineering Technology Research Center of Taxus,Sichuan Agricultural University,Chengdu 611130,Sichuan,China;Guangan Forestry Bureau,Guangan 638000,Sichuan,China)

机构地区：[1]四川农业大学国家林业草原红豆杉西南工程技术研究中心,四川成都611130 [2]广安市林业局,四川广安638000

出　　处：《西北林学院学报》2025年第2期42-50,共9页Journal of Northwest Forestry University

基　　金：四川省博士后创新人才支持项目(031-2322999070)。

摘　　要：云南红豆杉是天然的抗癌植物,具有较高的药用价值。为探索WRKY转录因子在云南红豆杉次生代谢物合成过程中的作用,基于云南红豆杉基因组数据和5个组织(树皮、根、叶、茎、枝皮)的转录组数据,鉴定和分析WRKY基因家族。研究结果表明,共鉴定出了25个结构较为保守的WRKY基因,其中编码蛋白的氨基酸为157~726个,分子质量为18079.40~79474.03 u,等电点为5.44~9.51,不稳定数为39.97~64.58,脂肪指数为45.17~80.41,所有蛋白质的亚细胞定位都处于细胞核内;24个TyuWRKY(云南红豆杉WRKY)基因不均衡地分布于7条染色体上,另1个WRKY基因处于ctg100上。系统发育分析表明,25个TyuWRKY蛋白被划分为3大类:6个属于Ⅰ类,18个属于Ⅱ类,1个属于Ⅲ类;同一类中TyuWRKY蛋白的Motif分布也大体相同,且成员内含子数量介于1~7个,外显子数量介于2~8个。表达量分析结果显示,除TyuWRKY5基因只在根部表达外,所有TyuWRKY基因都在5个组织中表达;通过启动子顺式作用元件分析,TyuWRKY基因包含多个与激素、胁迫以及生长发育相关的元件。Taxus yunnanensis is a natural anticancer plant with high medicinal value.In order to explore the role of WRKY transcription factor in the synthesis of secondary metabolites of T.yunnanensis,the WRKY gene family was identified and analyzed based on the T.yunnanensis genome data and the transcriptome data of five tissues(stem bark,root,leaf,stem,and branch bark).The results showed that a total of 25 structurally more conserved WRKY genes were identified,which encoded proteins with 157-726 amino acids,molecular mass of 18079.40-79474.03 u,isoelectric point of 5.44-9.51,instability number of 39.97-64.58,and fat index of 45.17-80.41,and all the proteins with sub cellular localisation were in the nucleus;24 TyuWRKY(T.yunnanensis WRKY)genes showed an uneven distribution on 7 chromosomes,another WRKY gene on ctg100;after phylogenetic analyses,the 25 TyuWRKY proteins were classified into 3 major classes:6 belonged to classⅠ,18 belonged to classⅡ,and 1 belonged to classⅢ;within the same class,the distribution of Motifs of TyuWRKY proteins in the same class was also largely the same,and the number of member introns ranged from 1 to 7,and the number of exons ranged from 2 to 8;according to the analysis of the expression amount,except TyuWRKY5 gene,which was expressed only in the roots,all of the TyuWRKY genes were expressed in five tissues;by the analysis of promoter cis-acting elements,TyuWRKY contained multiple genes related to hormones,stress,and growth and development.

关 键 词：云南红豆杉 WRKY转录因子 基因家族鉴定 表达分析 

分 类 号：S791.49[农业科学—林木遗传育种] Q786[农业科学—林学]