负载蜂胶的聚乙烯醇缩丁醛纳米纤维膜的制备及其性能  

Preparation of polyvinyl butyral nanofiber membrane loaded with propolis and its property

作　　者：代智慧 罗云纲 刘志辉[1] DAI Zhihui;LUO Yungang;LIU Zhihui(Department of Prosthodontics,Stomatology Hospital,Jilin University,Changchun 130021,China;Department of Stomatology,Jingyue Campus,First Hospital,Jilin University,Changchun 130117,China)

机构地区：[1]吉林大学口腔医院修复科,吉林长春130021 [2]吉林大学第一医院净月分院口腔科,吉林长春130117

出　　处：《吉林大学学报(医学版)》2025年第1期238-244,共7页Journal of Jilin University:Medicine Edition

基　　金：吉林省科技厅科技发展计划医药健康领域项目(20220204124YY);中国工程院战略研究与咨询项目(JL2023-18)。

摘　　要：目的:制备负载蜂胶的聚乙烯醇缩丁醛(PVB)纳米纤维膜,阐明其理化性能、药物释放行为、抗菌性和生物相容性。方法:将质量分数为0.12%的蜂胶溶解于质量分数为18%的PVB甲醇溶液中,利用静电纺丝法分别以PVB和PVB-蜂胶(PVB-P)溶液制备PVB及PVB-P纳米纤维膜;扫描电子显微镜(SEM)观察PVB-P纳米纤维微观形貌,Nano Measurer软件分析纤维直径分布,傅里叶变换红外光谱(FTIR)分析PVB-P化学组成,水接触角测量评价PVB-P亲水性,紫外分光光度计检测不同时间点蜂胶累计释放量;将PVB和PVB-P纳米纤维膜与金黄色葡萄球菌(S.aureus)、大肠杆菌(E.coli)、白色念珠菌(C.albicans)、沙门氏菌(Salmonella)及铜绿假单胞菌(P.aeruginosa)共培养,分为PVB组和PVB-P组,采用吸收法计算PVB和PVB-P对5种细菌的抑菌值;将NIH-3T3细胞接种于PVB和PVB-P纳米纤维膜上,分为PVB组和PVB-P组,CCK-8法检测PVB组和PVB-P组纳米纤维膜在1、3和7 d的细胞存活率。结果:SEM观察,PVB与PVB-P纳米纤维相互交叉呈网状多孔结构,粗细均匀,无串珠;Nano Measurer软件检测,PVB纳米纤维直径为(0.50±0.10)μm,PVB-P纳米纤维直径为(0.54±0.16)μm。FTIR分析,PVB-P纳米纤维膜出现PVB特征峰(1140和1002 cm^(-1))和蜂胶特征峰(1161 cm^(-1))。水接触角测量PVB膜为(144.26°±2.90°),PVB-P膜为(128.13°±1.36°)。紫外分光光度计检测,蜂胶1 d时释放0.04 mg,3 d时释放0.07 mg,7 d释放达到稳定,累计释放0.79 mg。吸收法检测,PVB-P纳米纤维膜对S.aureus、E.coli、C.albicans、Salmonella和P.aeruginosa抑菌值分别为4.39、1.27、5.68、3.16及1.87。CCK-8法检测,1、3和7 d NIH-3T3细胞在PVB组和PVB-P组细胞存活率均>90%,且组间比较差异无统计学意义(P>0.05)。结论:负载蜂胶的PVB纳米纤维膜直径增大,对S.aureus和C.albicans等具有抗菌作用并可实现蜂胶的持续释放。Objective:To prepare a polyvinyl butyral(PVB)nanofiber membrane loaded with propolis,and to clarify its physicochemical properties,drug release behavior,antibacterial activity,and biocompatibility.Methods:Propolis with a mass fraction of 0.12%was dissolved in 18%(mass fraction)PVB methanol solution.PVB and PVB-propolis(PVB-P)nanofiber membranes were prepared using electrospinning from PVB and PVB-P solutions,respectively.Scanning electron microscope(SEM)was used to observe the microscopic morphology of PVB-P nanofibers,and the Nano Measurer software was used to analyze the fiber diameter distribution.Fourier transform infrared spectroscopy(FTIR)was used to analyze the chemical composition of PVB-P.Water contact angle measurements were used to evaluate the hydrophilicity of PVB-P.UV spectrophotometer was used to detect the cumulative release of propolis at different time points.PVB and PVB-P nanofiber membranes were co-cultured with Staphylococcus aureus(S.aureus),Escherichia coli(E.coli),Candida albicans(C.albicans),Salmonella,and Pseudomonas aeruginosa(P.aeruginosa)and divided into PVB group and PVB-P group.The absorbance method was used to calculate the antibacterial values of PVB and PVB-P against the five types of bacteria.The NIH-3T3 cells were seeded on PVB and PVB-P nanofiber membranes and divided into PVB group and PVB-P group.The CCK-8 method was used to detect the survival rates of NIH-3T3 cells on the nanofiber membranes in PVB group and PVB-P group at 1,3,and 7 d.Results:The SEM results showed that PVB and PVB-P nanofibers were interconnected in a mesh-like porous structure,with uniform thickness and no beads.The Nano Measurer software measurement results showed that the diameter of PVB nanofibers was(0.50±0.10)μm,and the diameter of PVB-P nanofibers was(0.54±0.16)μm.FTIR analysis showed that PVB-P nanofiber membranes exhibited characteristic peaks of PVB(1140 and 1002 cm^(-1))and propolis(1161 cm^(-1)).The water contact angle measurement results showed that the contact angle of PVB membrane was(1

关 键 词：聚乙烯醇缩丁醛 蜂胶 静电纺丝 生物相容性 抗菌性能 

分 类 号：R318.08[医药卫生—生物医学工程]