Effect of Jinfeng Pill on ovarian function of Tripterygium wilfordii polyglycoside tablets induced poor ovarian response rats  

作　　者：Yi Li Jian-Fang Wang Xiao-Hong Yan Xiao-Lei Ji Heng-Yuan Zhang Rong-Feng Wu You-Zhu Li 

机构地区：[1]Clinical Medical Research Center for Obstetrics and Gynecology Diseases of Fujian Province,Laboratory of Research and Diagnosis of Gynecological Diseases of Xiamen City,Department of Reproductive Medicine,the First Afffliated Hospital of Xiamen University,School of Medicine,Xiamen University,Xiamen 361003,China [2]Internal Medicine,Siming Branch,The First Afffliated Hospital of Xiamen University,School of Medicine,Xiamen University,Xiamen 361005,China [3]Xiamen Assisted Reproductive Technology Quality Control Center,Xiamen 361000,China [4]School of Public Health,Xiamen University,Xiamen 361102,China [5]School of Medicine,Huaqiao University,Quanzhou 362000,China.

出　　处：《Reproductive and Developmental Medicine》2024年第4期206-212,共7页生殖与发育医学(英文版)

基　　金：Tong Yi Tang Pharmaceutical Co.,Ltd.,Wu Jieping Foundation Clinical Research Special Project(320.6750.2021-19-2);Fujian Province Natural Science Foundation Project(2023J011619);Xiamen Science and Technology Program(No.3502Z20227342)。

摘　　要：Objective:This study aimed to assess the effects of the Jinfeng pill on ovarian volume and endocrine function in rats with poor ovarian response and to elucidate the gene expression changes that contribute to the Jinfeng pill’s role in improving ovarian function.Methods:Thirty 8-week-old specific pathogen-free(SPF)female rats were randomly assigned to five groups:control,model,estradiol,Jinfeng pill,and Jinfeng pill+estradiol,with six rats in each group.All groups,except the control were administered Tripterygium glycoside tablets.After establishing the poor ovarian response model,the three intervention groups(estradiol,Jinfeng pill,and Jinfeng pill+estradiol)received their respective drug interventions.Hematoxylin-eosin staining was employed to assess follicle distribution,and immunohistochemistry was used to evaluate the expression of vascular endothelial growth factor and endostatin in rat ovaries.Hormone levels were measured using ELISA.Results:The uterine and total ovarian masses in the three intervention groups were higher than those in the model group;however,the differences were not statistically significant.The ovaries of the Jinfeng pill and Jinfeng pill+estradiol groups contained more follicles at various developmental stages.The three intervention groups showed significantly increased vascular endothelial growth factor expression and decreased endostatin expression compared with the model group,with the Jinfeng pill+estradiol group exhibiting the most pronounced differences.Anti-Müllerian hormone levels were slightly elevated in the Jinfeng pill group compared to the model group.However,only the Jinfeng pill+estradiol group showed a statistically significant decrease in follicle-stimulating hormone levels compared to the model group(P=0.01).Conclusions:Jinfeng pill modulates ovarian protein expression,increases ovarian volume,and improves blood circulation.Additionally,it may elevate hormone levels and enhance ovarian response function in rats with poor ovarian function.

关 键 词：Poor ovarian response Jinfeng Pill Sex hormone Vascular endothelial growth factor ENDOSTATIN 

分 类 号：R285.5[医药卫生—中药学]