磷酸三(2-氯乙基)酯暴露对海水青鳉胚胎转录组的影响  

Effects of tris(2-chloroethyl)phosphate exposure on the transcriptome of marine medaka embryos

作　　者：杜丽超 王婷 徐怡然 高东旭 雷威 DU Li-chao;WANG Ting;XU Yi-ran;GAO Dong-xu;LEI Wei(College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China;National Marine Environmental Monitoring Center,Dalian 116023,China)

机构地区：[1]大连海洋大学水产与生命学院,辽宁大连116023 [2]国家海洋环境监测中心,辽宁大连116023

出　　处：《环境生态学》2025年第2期153-158,共6页Environmental Ecology

基　　金：国家自然科学基金(42006126);辽宁省教育厅高等学校基本科研项目(LJKMZ20221088)资助。

摘　　要：磷酸三(2-氯乙基)酯(TCEP)是一种海洋新污染物,为探究其对海水青鳉胚胎转录水平的影响,本研究对暴露于0μg/L和25μg/L TCEP 10 d后的海水青鳉胚胎进行了转录组分析,并探讨了TCEP对海水青鳉发育的潜在毒性作用和可能的毒性机制。转录组分析结果显示,共获得了154个差异表达基因,其中上调基因74个,下调基因80个。差异基因主要富集到与神经、骨骼、视觉和生殖系统发育相关通路,包括神经发育、生长发育、骨生长和视黄醇结合、背-腹轴形成、雌激素应答、内质网蛋白加工和细胞衰老等通路。本研究结果为后续深入探究TCEP对海洋鱼类的发育毒性及机制提供了基础数据。Tris(2-chloroethyl)phosphate(TCEP)is an emerging marine pollutant.To investigate the effect of TCEP on the transcript levels of marine medaka embryos,we conducted a transcriptomic analysis of the marine medaka embryos exposed to 0μg/L and 25μg/L TCEP for 10 d.Furthermore,the potential toxic effects and mechanisms of TCEP on the development of marine medaka were discussed.A total of 154 differentially expressed genes were identified,including 74 up-regulated genes and 80 down-regulated genes.These differentially expressed genes were primarily enriched in development-related pathways,including those associated with the nervous,skeletal,vision,and reproductive systems.These pathways encompass neural development,developmental growth,bone growth,retinal binding,dorsal-ventral axis formation,response to estrogen,protein processing in the endoplasmic reticulum,and cellular senescence.These findings provide foundational data for subsequent in-depth investigations into the developmental toxicity and mechanisms of TCEP on marine fish.

关 键 词：海洋新污染物 海水青鳉 胚胎 TCEP 转录组测序 

分 类 号：X503.2[环境科学与工程—环境工程] X171.5