Extended-depth of field random illumination microscopy,EDF-RIM,provides super-resolved projective imaging  

作　　者：Lorry Mazzella Thomas Mangeat Guillaume Giroussens Benoit Rogez Hao Li Justine Creff Mehdi Saadaoui Carla Martins Ronan Bouzignac Simon Labouesse Jérome Idier Frédéric Galland Marc Allain Anne Sentenac Loïc LeGoff 

机构地区：[1]Aix Marseille Université,CNRS,Centrale Med,Institut Fresnel UMR7249,Turing Center for Living Systems,Marseille,France [2]LITC Core Facility,Centre de Biologie Integrative(CBI),CNRS,Universitéde Toulouse,UT3,Toulouse,France [3]MCD,Centre de Biologie Intégrative(CBI),CNRS,Universitéde Toulouse,UT3,Toulouse,France [4]Aix Marseille University,CNRS,IBDM UMR7288,TuringCentre for Living Systems,Marseille,France [5]LS2N,CNRS UMR 6004,F44321 Nantes Cedex 3,France

出　　处：《Light(Science & Applications)》2024年第12期3052-3063,共12页光(科学与应用)(英文版)

基　　金：support and fruitful discussions on the project.Figure S3A was created with BioRender.com;funded by the following agencies:Agence Nationale de la Recherche(ANR-18-CE13-028,ANR-20-CE45-0024,ANR-22-CE13-0039,ANR-22-CE42-0010,ANR-22-CE42-0026);Institut Carnot star(3D-RIM);funded by the《France 2030》investment plan managed by the French National Research Agency(ANR-16-CONV-0001,ANR-21-ESRE-0002);from Excellence Initiative of Aix-Marseille University-A*MIDEX.

摘　　要：The ultimate aim of fluorescence microscopy is to achieve high-resolution imaging of increasingly larger biological samples.Extended depth of field presents a potential solution to accelerate imaging of large samples when compression of information along the optical axis is not detrimental to the interpretation of images.We have implemented an extended depth of field(EDF)approach in a random illumination microscope(RIM).RIM uses multiple speckled illuminations and variance data processing to double the resolution.It is particularly adapted to the imaging of thick samples as it does not require the knowledge of illumination patterns.We demonstrate highly-resolved projective images of biological tissues and cells.Compared to a sequential scan of the imaged volume with conventional 2D-RIM,EDF-RIM allows an order of magnitude improvement in speed and light dose reduction,with comparable resolution.As the axial information is lost in an EDF modality,we propose a method to retrieve the sample topography for samples that are organized in cell sheets.

关 键 词：ILLUMINATION PROJECTIVE RESOLVED 

分 类 号：O15[理学—数学]