Long-range enhancement for fluorescence and Raman spectroscopy using Ag nanoislands protected with column-structured silica overlayer  

作　　者：Takeo Minamikawa Reiko Sakaguchi Yoshinori Harada Hiroki Tanioka Sota Inoue Hideharu Hase Yasuo Mori Tetsuro Takamatsu Yu Yamasaki Yukihiro Morimoto Masahiro Kawasaki Mitsuo Kawasaki 

机构地区：[1]Department of Systems Innovation,Graduate School of Engineering Science,Osaka University,Osaka 560-8531,Japan [2]Division of Interdisciplinary Researches for Medicine and Photonics,Institute of Post-LED Photonics,Tokushima University,Tokushima 770-8506,Japan [3]Department of Pathology and Cell Regulation,Graduate School of Medical Science,Kyoto Prefectural University of Medicine,Kyoto 602-8566,Japan [4]PRESTO,Japan Science and Technology Agency(JST),Tokushima 770-8506,Japan [5]Institute for Integrated Cell-Material Sciences(iCeMS),Kyoto University,Kyoto 606-8501,Japan [6]Department of Synthetic Chemistry and Biological Chemistry,Graduate School of Engineering,Kyoto University,Kyoto 615-8510,Japan [7]Department of Medical Photonics,Graduate School of Medical Science,Kyoto Prefectural University of Medicine,Kyoto 602-8566,Japan [8]Technology and Engineering Division,Ushio Inc.,Hyogo 671-0224,Japan [9]The Institute of Science and Industrial Research,Osaka University,Osaka 567-0047,Japan [10]Department of Molecular Engineering,Graduate School of Engineering,Kyoto University,Kyoto 615-8510,Japan

出　　处：《Light(Science & Applications)》2024年第12期3158-3170,共13页光(科学与应用)(英文版)

基　　金：supported by the PRESTO program(JPMJPR17PC)of Japan Science and Technology Agency(JST),Japan,JSPS KAKENHI(15K12519,19K22969,21H01847,22H00303)from the Japan Society for the Promotion of Science(JSPS),Japan,a research grant from the Research Clusters program of Tokushima University(1802003);support from Mr K.Hayashi(Kyoto University),Mr A.Aiba,Mr T.Saito,Ms H.Miura(Ushio Inc.),and Mr S.Sugano(Tokushima University)。

摘　　要：We demonstrate long-range enhancement of fluorescence and Raman scattering using a dense random array of Ag nanoislands(AgNIs)coated with column-structured silica(CSS)overlayer of over 100 nm thickness,namely,remote plasmonic-like enhancement(RPE).The CSS layer provides physical and chemical protection,reducing the impact between analyte molecules and metal nanostructures.RPE plates are fabricated with high productivity using sputtering and chemical immersion in gold(I)/halide solution.The RPE plate significantly enhances Raman scattering and fluorescence,even without proximity between analyte molecules and metal nanostructures.The maximum enhancement factors are 10^(7)-fold for Raman scattering and 102-fold for fluorescence.RPE is successfully applied to enhance fluorescence biosensing of intracellular signalling dynamics in HeLa cells and Raman histological imaging of oesophagus tissues.Our findings present an interesting deviation from the conventional near-field enhancement theory,as they cannot be readily explained within its framework.However,based on the phenomenological aspects we have demonstrated,the observed enhancement is likely associated with the remote resonant coupling between the localised surface plasmon of AgNIs and the molecular transition dipole of the analyte,facilitated through the CSS structure.Although further investigation is warranted to fully understand the underlying mechanisms,the RPE plate offers practical advantages,such as high productivity and biocompatibility,making it a valuable tool for biosensing and biomolecular analysis in chemistry,biology,and medicine.We anticipate that RPE will advance as a versatile analytical tool for enhanced biosensing using Raman and fluorescence analysis in various biological contexts.

关 键 词：structure SILICA protected 

分 类 号：O62[理学—有机化学]