Fresenius COM. TEC与Spectra OPTIA采集外周血造血干细胞的特点及其临床应用  

Characteristics and Clinical Application of Fresenius COM.TEC and Spectra OPTIA in Collection of Peripheral Blood Hema-topoietic Stem Cells

作　　者：李少文[1] 朱展鸿 高旖鑫 王刚 郭楚霞 陆志刚[1] 彭永正[1] LI Shaowen;ZHU Zhanhong;GAO Yirin;WANG Gang;GUO Churia;LU Zhigang;PENG Yongzheng(Department of Trans fusion Medicine,Zhujiang Hospital Affiliated to Southern Medical University,Guangzhou Guangdomg510000,China)

机构地区：[1]南方医科大学珠江医院输血医学科,广东广州510000 [2]南部战区总医院输血医学科

出　　处：《联勤军事医学》2024年第12期1064-1068,共5页Military Medicine of Joint Logistics

摘　　要：目的 比较Fresenius COM.TEC与Spectra OPTIA两种血细胞分离机在采集捐献者外周血造血干细胞时的采集效率、采集物特性及对捐献者后续的影响。方法 回顾性分析2020-03/2023-10月在作者医院接受外周血造血干细胞采集的217例健康捐献者资料,包括采集前后捐献者的血常规、电解质、采集过程中的相关参数、不良反应情况和采集物单个核细胞数及CD34^(+)细胞数。结果 Fresenius COM.TEC与Spectra OPTIA在采集外周血造血干细胞后对捐献者的血常规和电解质指标、采集过程中的循环血量、采集效率、采集物的单个核细胞数、CD34^(+)细胞数、采集过程及采集后不良反应发生率比较差异无统计学意义(P>0.05),但在血液保存液用量上,Fresenius COM.TEC比Spectra OPTIA更少(P<0.05)。而Fresenius COM.TEC采集的产物中,白细胞计数、红细胞计数、红细胞比容、血小板计数及中性粒细胞计数均显著高于Spectra OPTIA(P均<0.05)。结论 Fresenius COM.TEC与Spectra OPTIA两种血细胞分离机均能有效且安全地完成外周血造血干细胞的采集任务。在实际应用中,可根据捐献者的具体特点选择合适的机器或调整动员方案,以达到最佳的采集效果。Objective To compare the collection efficiency,collection characteristics,and follow-up effects of Fresenius COM.TEC and Spectra OPTIA blood cell separators in the collection of peripheral blood hematopoietic stem cells from donors.Methods A total of 217 healthy donors who received peripheral blood hematopoietic stem cell collection in author's hospital from March 2020 to October 2023 were retrospectively analyzed,including blood routine before and after the collection,electrolyte detection,relevant parameters during the collection,adverse reactions,mononuclear cell number and CD34^(+)cell number in the collection.Results There were no significant differences between the two instruments in blood routine and electrolyte indexes of donors,circulating blood volume during collection,collection efficiency,number of mononuclear cells and CD34^(+)cells,and the incidence of adverse reactions during and after collection(P>0.05),but Fresenius COM.TEC used less blood maintenance fluid than that of Spectra OPTIA(P<0.05).In addition,the total number of white blood cells,the number of red blood cells,the specific volume of red blood cells,the number of platelet and the number of neutrophil in the products of Fresenius COM.TEC were significantly higher than those of Spectra OPTIA(all P<0.05).Conclusion Both Fresenius COM.TEC and Spectra OPTIA blood cell separators can effectively and safely complete the collection of peripheral blood hematopoietic stem cells.In practical application,the appropriate machine or mobilization scheme can be selected according to the specific characteristics of the donors to achieve the best collectioneffect.

关 键 词：Fresenius COM.TEC Spectra OPTIA 外周血造血干细胞 血细胞分离机 CD34^(+)细胞 

分 类 号：R446.11[医药卫生—诊断学]