荧光法用于体液中微量氯离子检测研究进展  

Research Progress of Fluorescence Methods for Detection of Trace Chloride Ion in the Body Fluids

作　　者：陈文博 赵留创 张雪芳 胡乐乾[1] CHEN Wenbo;ZHAO Liuchuang;ZHANG Xuefang;HU Leqian(School of Chemistry and Chemical Engineering,Henan University of Technology,Zhengzhou 450001)

机构地区：[1]河南工业大学化学化工学院,河南郑州450001

出　　处：《分析科学学报》2025年第1期90-98,共9页Journal of Analytical Science

基　　金：河南工业大学自科创新基金(2022ZKCJ08);河南工业大学高层次人才科研启动基金项目(2021BS052)。

摘　　要：人体体液中微量氯离子的定量检测对于疾病预防和诊断具有重要意义。荧光分析法具有高灵敏度、高选择性和良好的时空分辨率等优势,在体液中氯离子检测方面有广阔的应用前景。本文基于荧光分子发光机理,将近十年用于氯离子检测的荧光分析方法总结为四类:碰撞猝灭、氢键作用、配体位移和荧光分子分解。详细阐述了各种荧光分析方法对氯离子定量检测的原理,讨论了它们的优缺点和适用范围。The quantitative detection of trace chloride ion in body fluids is significant for disease prevention and diagnosis.With the advantages of high sensitivity,high selectivity and good temporal and spatial resolution,fluorescence analysis has a broad application prospect in detecting chloride ion in body fluids.Based on the fluorescence emission mechanisms of fluorophores,this paper summarizes the fluorescence analysis methods used for chloride ion detection over the past decade into four categories:collisional quenching,hydrogen bonding,ligand displacement,and fluorescent molecule decomposition.The principles of various fluorescence methods,and their advantages and disadvantages for the quantitative detection of chloride ions are described in detail.

关 键 词：氯离子 人体体液 荧光探针 检测指标 

分 类 号：O657.3[理学—分析化学]