高效液相色谱法测定天麻-川芎药对不同配伍比例中川芎嗪的含量  

HPLC Determination on the Content of Ligustrazine in Different Proportions of Gastrodia elata Blume and Ligusticum chuanxiong Hort

作　　者：吴丽宁[1] WU Li-ning(Pharmacy Department of the Second Affiliated Hospital of Guangxi Medical University,Nangning 532200,China)

机构地区：[1]广西医科大学第二附属医院药学部,广西南宁532200

出　　处：《海峡药学》2025年第1期10-13,共4页Strait Pharmaceutical Journal

基　　金：广西壮族自治区中医药管理局自筹经费科研课题(基金编号:GZZC2020194);基于代谢组学探讨川芎白芷对偏头痛大鼠作用机制研究(基金编号:EFYKY2020015)。

摘　　要：目的考察川芎配伍天麻后对川芎嗪检出含量的影响。方法通过高效液相法,对川芎嗪单药组和不同配伍比例的药对组进行川芎嗪的含量测定。采用色谱柱Venusil MP-C_(18)(4.6 mm×250 mm,5μm);以甲醇-水(50∶50,体积比)为流动相;检测波长:280 nm;柱温25℃,流速0.8 mL·min^(-1)。结果川芎嗪含量在0.30~20μg·mL^(-1)范围内与峰面积值呈良好线性关系(r=0.9994),精密度和重复性试验的RSD均<2.0%(n=6),稳定性试验结果显示各组样品在24 h内稳定,川芎组、天麻川芎A组、天麻川芎B组川芎嗪的平均回收率分别为:101.31%、103.49%、102.26%,RSD值分别为:1.90%,1.18%,1.79%。川芎组、天麻川芎A组、天麻川芎B组的川芎嗪检出含量依次为0.0743 mg·g^(-1)、0.095 mg·g^(-1)、0.0826 mg·g^(-1)。结论天麻川芎配伍有利于川芎嗪的提取,1∶4的配伍比例优于4∶1的配伍比例。OBJECTIVE Examining the compatibility of Gastrodia elata Blume and Ligusticum chuanxiong Hort affects the quantity of ligustrezine.METHODS The content of ligustrazine was determined in the single drug group and the drug pair group with different compatibility ratios by HPLC.The chromatographic separation was performed on an chromatographic column Venusil MP-C_(18)(4.6 mm×250 mm,5μm)with methanol-water(50∶50,volume ratio)as the mobile phase.The detection wavelength was 280 nm.The column temperature was kept at 25℃ and the flow rate was 0.8 mL·min^(-1).RESULTS The content of ligustrazine showed a good linear relationship with peak area values within the range of 0.30-20μg·mL^(-1)(r=0.9994),and the RSD of precision and repeatability tests were both<2.0%(n=6).The stability test results showed that the samples in each group were stable within 24 hours.The average recovery rates of Ligusticum chuanxiong Hort group and Gastrodia elata Blume and Ligusticum chuanxiong Hort group A(4∶1)and Gastrodia elata Blume and Ligusticum chuanxiong Hort group B(1∶4)were 101.31%,103.49%,and 102.26%,respectively,with RSD values were 1.90%,1.18%,and 1.79%.The detected levels of ligustrazine content in the Ligusticum chuanxiong Hort group and Gastrodia elata Blume and Ligusticum chuanxiong Hort group A(4∶1)and Gastrodia elata Blume and Ligusticum chuanxiong Hort group B(1∶4)were 0.0743 mg·g^(-1),0.095 mg·g^(-1)and 0.0826 mg·g^(-1),respectively.CONCLUSION The Gastrodia Chuanxiong couplet medicines compatibility is conducive for ligustrazine extraction,and a 1∶4 ratio of compatibility is better than 4∶1 ratio.

关 键 词：天麻 川芎 高效液相 川芎嗪 

分 类 号：R927[医药卫生—药学]