A simple,highly efficient Agrobacterium tumefaciensmediated moss transformation system with broad applications  

作　　者：Ping Zhou Xiujin Liu Yuqing Liang Yan Zhang Xiaoshuang Li Daoyuan Zhang 

机构地区：[1]State Key Laboratory of Desert and Oasis Ecology,Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands,Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China [2]Department of Food Science and Engineering,Moutai Institute,Renhuai 564502,China [3]Xinjiang Key Lab of Conservation and Utilization of Plant Gene Resources,Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China [4]University of Chinese Academy of Sciences,Beijing 100049,China [5]Shanghai Center for Plant Stress Biology,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China

出　　处：《aBIOTECH》2024年第4期476-487,共12页生物技术通报(英文版)

基　　金：supported by the Leading Talents in Technological Innovation programme(2022TSYCLJ0049);the Second Tibetan Plateau Scientific Expedition and Research(STEP)program(2019QZKK0502030403).

摘　　要：Mosses,particularly desiccation-tolerant(DT)species,are important model organisms for studying genes involved in plant development and stress resistance.The lack of a simple and efficient stable moss transformation system has hindered progress in deciphering the genetic mechanisms underlying traits of interest in these organisms.Here,we present an Agrobacterium tumefaciens-mediated transformation system for DT mosses that uses Agrobacterium strain EHA105 harboring the binary vector pCAMBIA1301-GUS.This system achieved transformation efficiencies of 74%and 81%in Physcomitrium patens and Bryum argenteum protonemata,respectively,without the need for culture and callus formation prior to regeneration.We detected GUS enzyme activity in the regenerated transgenic moss via histochemical staining.Southern blot,PCR,and RT-qPCR analyses confirmed the presence of the GUS gene.In addition,we successfully used this system to transform wild DT Syntrichia caninervis.Furthermore,P.patens and B.argenteum transformed using this system with the stress resistance gene EsDREB from the desert plant Eremosparton songoricum(Litv.)exhibited improved salt tolerance.We thus present an efficient tool for the genetic analysis of DT moss species,paving the way for the development of stress-resistant crop cultivars.

关 键 词：Agrobacterium tumefaciens Highly efficient MOSS TRANSFORMATION 

分 类 号：Q94[生物学—植物学]