人工虎骨粉联合GelMA水凝胶可促进退变椎间盘再生  

Bionic Tiger Bone Powder Combined with GelMA Hydrogel Can Promote the Regeneration of Degenerative Intervertebral Disc

作　　者：张永峰 高荣[2] 李盼 张鹏 潘东晟 张家玮 丁坦 ZHANG Yong-feng;GAO Rong;LI Pan;ZHANG Peng;PAN Dong-sheng;ZHANG Jia-wei;DING Tan(Department of Orthopaedics,The First Affiliated Hospital of Air Force Military Medical University,Xi'an,Shaanxi,710032,China;Research Center of Bioengineering and Mechanics,Xi'an Jiaotong University,Xi'an,Shaanxi,710049,China)

机构地区：[1]空军军医大学第一附属医院骨科,陕西西安710032 [2]西安交通大学生物工程与力学研究中心,陕西西安710049

出　　处：《现代生物医学进展》2025年第1期36-45,共10页Progress in Modern Biomedicine

基　　金：陕西省重点研发计划项目(2023-ZDLSF-12)。

摘　　要：目的:探究负载人工虎骨粉的可注射甲基丙烯酸明胶(GelMA)水凝胶支架对大鼠椎间盘退变的修复效果。方法:分别合成3%、4%和5%的复合GelMA水凝胶支架,并表征微观结构,利用力学流变仪观察水凝胶基质刚度的变化。采用细胞染色技术和CCK-8观察髓核细胞在负载人工虎骨粉的水凝胶支架中的生长形态并对其增殖能力进行评估,随后采用ELISA检测细胞炎症反应。构建大鼠尾椎椎间盘退变模型,注射该水凝胶支架,通过CT扫描和免疫荧光染色评估水凝胶支架对椎间盘退变的修复效果。结果:成功构建了基质刚度可调控的GelMA水凝胶支架,该支架具有良好的生物相容性,体外实验发现人工虎骨粉可以明显促进细胞增殖,减弱细胞炎症反应。在体内研究中,该水凝胶支架显著恢复椎间盘高度,改善炎性微环境并促进髓核再生。结论:负载人工虎骨粉的GelMA水凝胶支架具有良好的机械性能和生物相容性,可以通过改善炎性微环境和力学微环境双重作用修复退变椎间盘。Objective:To investigate the repair effect of injectable GelMA hydrogel scaffold loaded with bionic tiger bone powder on intervertebral disc degeneration in rats.Methods:We synthesized composite GelMA hy-drogels with concentrations of 3%,4%,and 5%,characterized their microstructure,and observed changes in the stiffness of the hydrogel matrix using a rheometer.We used cell staining techniques and CCK-8 assays to observe the growth morphology and assess proliferation of nucleus pulposus cells in hydrogel scaffolds loaded with bionic tiger bone powder,followed by ELISA to detect cellular inflammatory responses.We then established a rat caudal inter-vertebral disc degeneration model and injected the hydrogel scaffolds,evaluating the scaffolds'repair effects on disc degeneration using CT scans and immunofluorescence staining.Results:We have successfully developed a GelMA hydrogel scaffold with adjustable matrix stiffness,which exhibits excelle nt biocompatibility.In vitro experiments have shown that bionic tiger bone powder can significantly promote cell proliferation and attenuate cell inflammatory responses.In vivo studies have demonstrated that the hydrogel scaffold significantly restores disc height,improves the inflammatory microenvironment and promotes nucleus pulposus regeneration.Conclusions:GelMA hydrogel scaffold loaded with bionic tiger bone powder has good mechanical properties and biocompatibility,and can repair degenerated intervertebral disc by improving both inflammatory microenvironment and mechanical microenviron-ment.

关 键 词：椎间盘退变 人工虎骨粉 GelMA水凝胶 炎症反应 

分 类 号：R3[医药卫生—基础医学] R687.3