Qishen Granules Modulate Metabolism Flexibility Against Myocardial Infarction via HIF-1α-Dependent Mechanisms in Rats  

作　　者：SUN Xiao-qian LI Xuan LI Yan-qin LU Xiang-yu LIU Xiang-ning CUI Ling-wen WANG Gang ZHANG Man LI Chun WANG Wei 

机构地区：[1]School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing,100029,China [2]Beijing Key Laboratory of Traditional Chinese Medicine Syndrome and Formula,Beijing University of Chinese Medicine,Beijing,100029,China [3]School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing,100029,China [4]Guangzhou University of Chinese Medicine,Guangdong,510006,China

出　　处：《Chinese Journal of Integrative Medicine》2025年第3期215-227,共13页中国结合医学杂志(英文版)

基　　金：Supported by the National Natural Science Foundation of China(No.82174215);the National Science Fund for Excellent Young Scholars(No.82222075);the National Major Scientific and Technological Special Project for"Significant New Drugs Development"(No.2019ZX09201004-001-011)。

摘　　要：Objective:To assess the cardioprotective effect and impact of Qishen Granules(QSG)on different ischemic areas of the myocardium in heart failure(HF)rats by evaluating its metabolic pattern,substrate utilization,and mechanistic modulation.Methods:In vivo,echocardiography and histology were used to assess rat cardiac function;positron emission tomography was performed to assess the abundance of glucose metabolism in the ischemic border and remote areas of the heart;fatty acid metabolism and ATP production levels were assessed by hematologic and biochemical analyses.The above experiments evaluated the cardioprotective effect of QSG on left anterior descending ligation-induced HF in rats and the mode of energy metabolism modulation.In vitro,a hypoxia-induced H9C2 model was established,mitochondrial damage was evaluated by flow cytometry,and nuclear translocation of hypoxia-inducible factor-1α(HIF-1α)was observed by immunofluorescence to assess the mechanism of energy metabolism regulation by QSG in hypoxic and normoxia conditions.Results:QSG regulated the pattern of glucose and fatty acid metabolism in the border and remote areas of the heart via the HIF-1αpathway,and improved cardiac function in HF rats.Specifically,QSG promoted HIF-1αexpression and entry into the nucleus at high levels of hypoxia(P<0.05),thereby promoting increased compensatory glucose metabolism;while reducing nuclear accumulation of HIF-1αat relatively low levels of hypoxia(P<0.05),promoting the increased lipid metabolism.Conclusions:QSG regulates the protein stability of HIF-1α,thereby coordinating energy supply balance between the ischemic border and remote areas of the myocardium.This alleviates the energy metabolism disorder caused by ischemic injury.

关 键 词：Qishen Granules substrate utilization hypoxia-inducible factor-1αpathway energy metabolism heart failure 

分 类 号：R285.5[医药卫生—中药学]