胚蛋注射鼠李糖乳杆菌对黄羽肉鸡早期生长性能、肠道健康、血清生化指标和盲肠微生物区系的影响  

Effects of in Ovo Injection of Lactobacillus rhamnosus on Growth Performance,Intestinal Health,Serum Biochemical Indices and Cecal Microflora of Yellow-Feathered Broilers at Early Stage

作　　者：于潇 姚杰 苏琳洁 吴亚妮 马逸轩 黄艳玲[1] 王茜 YU Xiao;YAO Jie;SU Linjie;WU Yani;MA Yixuan;HUANG Yanling;WANG Xi(Key Laboratory of Animal Science of National Ethnic Affairs Commission,Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resources Reservation and Utilization,College of Animal and Veterinary Sciences,Southwest Minzu University,Chengdu 610041,China)

机构地区：[1]西南民族大学畜牧兽医学院,青藏高原动物遗传资源保护与利用教育部重点实验室,动物科学国家民委重点实验室,成都610041

出　　处：《动物营养学报》2025年第2期938-949,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(32102591);西南民族大学中央高校基本科研业务费专项资金项目(ZYN2023002)。

摘　　要：本试验旨在研究胚蛋注射鼠李糖乳杆菌(Lactobacillus rhamnosus,LR)对黄羽肉鸡早期生长性能、肠道健康、血清生化指标和盲肠微生物区系的影响。采用单因子完全随机区组设计,选取240枚黄羽肉鸡种蛋[(58.70±0.25)g],随机放置于同一孵化器中8层(区组),每层设置3排。在18.5胚龄时,种蛋通过羊膜腔分别接种100μL磷酸盐缓冲液(对照组)、100μL低剂量LR(102 CFU,低LR组)和100μL高剂量LR(104 CFU,高LR组),每组8个重复,每个重复10枚蛋。出孵后,3组仔鸡按区组顺序转入对应鸡笼,饲喂相同饲粮至21日龄。每组8个重复(笼),每个重复9~10只鸡。结果显示:1)与对照组相比,高、低剂量LR组黄羽肉鸡21日龄体重与1~21日龄平均日增重均显著升高(P<0.05)。2)与对照组相比,胚蛋注射高、低剂量的LR均显著增加了黄羽肉鸡21日龄十二指肠、空肠、回肠绒毛高度(P<0.05)。3)与对照组相比,低剂量LR组血清白蛋白和总蛋白含量显著升高(P<0.05)。4)16S rRNA扩增子测序数据显示,胚蛋注射LR未显著影响黄羽肉鸡盲肠微生物α多样性(Shannon指数、Simpson指数、Chao指数、Sobs指数,P>0.05),但β多样性分析显示盲肠微生物群落结构发生显著变化(P<0.05)。与对照组相比,胚蛋注射高剂量LR显著升高了UCG-005属的相对丰度(P<0.05),胚蛋注射低剂量LR显著提高了蓝绿藻菌属的相对丰度(P<0.05)。综上所述,胚蛋注射LR(10^(2)或10^(4) CFU)可以通过改善肠道形态、增加肠道有益菌占比提高黄羽肉鸡早期的生长速度。This experiment was conducted to investigate the effects of in ovo injection of Lactobacillus rhamnosus(LR)on growth performance,intestinal health,serum biochemical indices and cecal microflora of yellow-feathered broilers at early stage.A randomized complete block design was applied in this study.A total of 240 hatching eggs of yellow-feathered broilers with similar weight[(58.70±0.25)g]were randomly allocated in the same incubator with eight layers(layer served as block).Each layer contained 3 rows of eggs.On 18.5 day of incubation,hatching eggs were randomly injected 100μL of phosphate buffer(control group),100μL of low dose LR(102 CFU,low-dose LR group),or 100μL of high dose LR(104 CFU,high-dose LR group)at amnion.There were 8 replicates in each group and 10 eggs in each replicate.At hatch,the in ovo injected birds were transferred to corresponding cage according the block order and continually fed the same diet until 21 days of age.There were 8 replicates(cages)in each group and 9 to 10 birds in each replicate.The results showed as follows:1)the body weight at 21 days of age and average daily gain from 1 to 21 days of age in both LR injected groups were significantly higher than those in the control group(P<0.05).2)The duodenal,jejunal and ileal villus heights and villus widths at 21 days of age in the LR injected groups were significantly increased compared with the groups(P<0.05).3)As compared to the control group,the contents of serum albumin and total protein in low-dose LR group were significantly increased(P<0.05).4)16S rRNA amplicon sequencing data indicated that in ovo injection of LR did not significantly affect cecal microbialαdiversity(Shannon index,Simpson index,Chao index and Sobs index,P>0.05).However,theβdiversity analysis indicated the cecal microbial structure changed significantly(P<0.05).Compared with the control group,the high dose LR injection significantly increased the relative abundance of UCG-005(P<0.05),and the low dose LR injection significantly increased the relative abundan

关 键 词：胚蛋注射 鼠李糖乳杆菌 黄羽肉鸡 生长性能 微生物区系 

分 类 号：S831[农业科学—畜牧学]