饲粮中添加葡萄籽原花青素对康乐黄公鸡睾丸氧化损伤的影响  

Effects of Dietary Grape Seed Proanthocyanidins on Testes Oxidative Damage of Kangle Yellow Roosters

作　　者：丁香 胡威 李子怡 徐德明 樊庆灿 杨雪 郑文亚[1,2] DING Xiang;HU Wei;LI Ziyi;XU Deming;FAN Qingcan;YANG Xue;ZHENG Wenya(College of Life Science and Resources and Environment,Yichun University,Yichun 336000,China;Engineering Technology Research Center of Jiangxi Universities and Colleges for Selenium Agriculture,Yichun 336000,China)

机构地区：[1]宜春学院生命科学与资源环境学院,宜春336000 [2]江西省高等学校硒农业工程技术研究中心,宜春336000

出　　处：《动物营养学报》2025年第2期1033-1046,共14页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金青年科学基金项目(32202762);江西省教育厅科学技术研究项目(GJJ211628,GJJ2201729);宜春学院引进高层次人才科研启动基金项目(3360119068)。

摘　　要：本试验旨在探究饲粮中添加葡萄籽原花青素(GSPs)对氧化应激条件下公鸡睾丸组织结构、抗氧化能力、抗凋亡能力和睾酮合成相关调控分子表达的影响。试验选取90只健康、体重相近的30日龄康乐黄公鸡,随机分成3组,每组3个重复,每个重复10只鸡。利用敌草快(DQ)作为氧化应激诱导剂。对照(CON)组和DQ组饲喂基础饲粮(不添加GSPs),GSPs+DQ组在基础饲粮中添加400 mg/kg的GSPs。各组饲喂相应饲粮30 d后,DQ组和GSPs+DQ组公鸡一次性腹腔注射25 mg/kg BW的DQ,对照组公鸡一次性腹腔注射等剂量的生理盐水,12 h后采集各组公鸡睾丸样品。预试期7 d,正试期31 d。结果显示:1)各组之间终末体重、平均日增重(ADG)、平均日采食量(ADFI)和料重比(F/G)均无显著差异(P>0.05)。2)与CON组相比,DQ组睾丸组织中核因子红细胞2相关因子2(Nrf2)的mRNA相对表达量显著上调(P<0.05),半氧合酶-1(HO-1)的mRNA相对表达量显著下调(P<0.05),过氧化氢酶(CAT)活性及丙二醛(MDA)含量均显著升高(P<0.05);与DQ组相比,GSPs+DQ组睾丸组织中Nrf2和谷胱甘肽过氧化物酶1(Gpx1)的mRNA相对表达量显著上调(P<0.05),总超氧化物歧化酶(T-SOD)活性显著升高(P<0.05),MDA含量显著降低(P<0.05)。3)与CON组相比,DQ组睾丸组织生精小管中的生精细胞排列略为稀疏,生精小管间的睾丸间质细胞密度仍维持在较高水平;饲粮中添加GSPs可有效缓解DQ对公鸡睾丸组织结构的氧化损伤。4)与CON组相比,DQ组睾丸组织中B细胞淋巴瘤-2相关X蛋白(Bax)、半胱氨酸天冬氨酸蛋白酶3(Caspase3)的mRNA相对表达量均显著上调(P<0.05),Bax的蛋白相对表达量也显著上调(P<0.05),B细胞淋巴瘤-2(Bcl-2)的蛋白相对表达量显著下调(P<0.05);与DQ组相比,GSPs+DQ组睾丸组织中Bax、Caspase3的mRNA和蛋白相对表达量显著下调(P<0.05),Bcl-2的mRNA和蛋白相对表达量均显著上调(P<0.05)。5)与CON组相比,DQ组睾丸组织中类固醇激素�This experiment was conducted to investigate the effects of dietary grape seed proanthocyanidins(GSPs)on testis tissue structure,antioxidant capacity,anti-apoptosis ability and the expression of testosterone synthesis-related regulatory molecules of roosters under oxidative stress conditions.A total of 90 healthy Kangle yellow roosters at 30 days of age with similar body weight were randomly divided into 3 groups with 3 replicates per group and 10 roosters per replicate.Diquat(DQ)was used as an oxidative stress inducer.Roosters in the control(CON)group and DQ group were fed a basal diet(without adding GSPs),and others in the GSPs+DQ group were fed the basal diet supplemented with 400 mg/kg GSPs.After feeding the corresponding feed to each group for 30 days,roosters in the DQ group and GSPs+DQ group were injected with 25 mg/kg BW of DQ intraperitoneally,and others in the control group were injected with an equal volume of physiological saline.Collected the testis samples from each group of roosters 12 h later.The pre-trial period was 7 days,and the experimental period was 31 days.The results showed as follows:1)there were no significant differences on the final body weight,average daily gain(ADG),average daily feed intake(ADFI)and feed-to-gain ratio(F/G)among all groups(P>0.05).2)Compared with the CON group,the mRNA relative expression level of nuclear factor erythroid 2-related factor 2(Nrf2)in testis tissue of the DQ group was significantly up-regulated(P<0.05),the haemoxygenase-1(HO-1)mRNA relative expression level was significantly down-regulated(P<0.05),and the catalase(CAT)activity and malondialdehyde(MDA)content were significantly increased(P<0.05);compared with the DQ group,the mRNA relative expression levels of Nrf2 and glutathione peroxidase 1(Gpx1)in testis tissue of the GSPs+DQ group were significantly up-regulated(P<0.05),the total superoxide dismutase(T-SOD)activity was significantly increased(P<0.05),and the MDA content was significantly decreased(P<0.05).3)Compared with the CON group,the arrangemen

关 键 词：葡萄籽原花青素 睾丸 组织结构 氧化应激 细胞凋亡 睾酮合成相关调控分子 

分 类 号：S831[农业科学—畜牧学]