Encapsulating lipase on the surface of magnetic ZIF-8 nanosphers with mesoporous SiO_(2)nano-membrane for enhancing catalytic performance  

作　　者：Guang-Xu Duan Queting Chen Rui-Rui Shao Hui-Huang Sun Tong Yuan Dong-Hao Zhang 

机构地区：[1]College of Pharmaceutical Science,Hebei University,Baoding 071002,China [2]Key Laboratory of Pharmaceutical Quality Control of Hebei Province,College of Pharmaceutical Science,Hebei University,Baoding 071002,China [3]Institute of Life Science and Green Development,Hebei University,Baoding 071002,China [4]Affiliated Hospital of Hebei University,Baoding 071000,China

出　　处：《Chinese Chemical Letters》2025年第2期251-255,共5页中国化学快报(英文版)

基　　金：the financial supports from the National Natural Science Foundation of China(Nos.22378093,21878065);Natural Science Foundation of Hebei Province,China(No.E2022201100);the Science and Technology Support Plan of Baoding City(No.2241ZF111);the Medical Science Foundation of Hebei University(No.2021A09);the Foundation of Affiliated Hospital of Hebei University(No.2021Z003)。

摘　　要：The preparation of immobilized enzyme with excellent performance is one of the difficulties that restrict the application of enzyme catalysis technology.Here,Candida rugosa lipase(CRL)was firstly adsorbed on the surface of magnetic zeolitic imidazolate framework-8(ZIF-8)nanospheres,which was further encapsulated with a mesoporous SiO_(2)nano-membrane formed by tetraethyl orthosilicate(TEOS)polycondensation.Consequently,lipase could be firmly immobilized on carrier surface by physical binding rather than chemical binding,which did not damage the active conformation of enzyme.There were mesopores on the silica nano-membrane,which could improve the accessibility of enzyme and its apparent catalytic activity.Moreover,silica membrane encapsulation could also improve the stability of enzyme,suggesting an effective enzyme immobilization strategy.It showed that TEOS amount and the encapsulation time had significant effects on the thickness of silica membrane and the enzyme activity.The analysis in enzyme activity and protein secondary structure showed that lipase encapsulated in silica membrane retained the active conformation to the greatest extent.Compared with the adsorbed lipase,the encapsulated lipase increased its thermostability by 3 times and resistance to chemical denaturants by 7 times.The relative enzyme activity remained around 80%after 8 repetitions,while the adsorbed lipase only remained at7.3%.

关 键 词：Enzyme immobilization ZIF-8 ENCAPSULATION Mesoporous silica membrane Lipase activity 

分 类 号：TQ426.97[化学工程]