可诱导驯化免疫的屎肠球菌分离筛选及其抗感染效果评价  

Isolation,screening,and anti-infectious evaluation of Enterococcus faecium capable of inducing trained immunity

作　　者：初红 王贺[2] 贾秀文 李娜 李丰阳 雷连成 CHU Hong;WANG He;JIA Xiuwen;LI Na;LI Fengyang;LEI Liancheng(State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases,Key Laboratory for Zoonosis Research of the Ministry of Education,Institute of Zoonosis,and College of Veterinary Medicine,Changchun 130062,Jilin,China;Department of Rehabilitation Medicine,The Second Hospital of Jilin University,Changchun 130062,Jilin,China)

机构地区：[1]吉林大学动物医学学院人畜共患传染病重症诊治全国重点实验室,吉林长春130062 [2]吉林大学第二医院康复医学中心,吉林长春130062

出　　处：《微生物学通报》2025年第2期785-798,共14页Microbiology China

基　　金：国家重点研发计划(2022YFD1800905)。

摘　　要：【背景】天然免疫应答作为机体的第一道免疫防御屏障,在动物抵抗病原微生物感染的初始阶段发挥着关键作用。从驯化免疫的角度,增强动物天然免疫,提高动物抗病能力是预防控制疾病的有效措施。【目的】从猪肠道菌群中筛选分离具有潜在驯化免疫作用的细菌,为挖掘新的免疫刺激物奠定基础。【方法】从健康猪粪便中分离纯化细菌,选择大蜡螟(Galleria mellonella)幼虫驯化免疫模型用于筛选肠道共生菌,对有潜在驯化免疫功能的细菌进行16S rRNA基因序列鉴定和生长曲线的测定,并利用小鼠模型对细菌进行体内驯化效果评价。【结果】通过使用大蜡螟幼虫模型,我们筛选出了一株具有诱导驯化免疫能力的屎肠球菌(Enterococcus faecium)SC-2;通过小鼠模型进一步证实菌株SC-2预刺激可以提高小鼠外周血与肺脏中的中性粒细胞和巨噬细胞占比,显著降低猪链球菌(Streptococcus suis)感染后其主要脏器中的菌载量,显著提高小鼠存活率。【结论】屎肠球菌SC-2具有诱导驯化免疫的作用,该菌株的分离鉴定为免疫调控新成分的挖掘提供了基础。[Background]The innate immune system is the first defense line and plays a key role in the host defense against microbial early infection.Enhancing the innate immunity from trained immunity is an effective way to improve the resistance of animals to diseases.[Objective]To isolate the bacteria capable of inducing trained immunity from healthy porcine intestinal flora and thus provide new insights for the prevention and control of microbial diseases by trained immunity.[Methods]Bacteria were isolated from healthy pig feces,and the strains capable of inducing trained immunity were screened with Galleria mellonella,a well-established model.The strain screened out was identified by 16S rRNA gene sequencing and characterized by growth curve.Its capability of inducing trained immunity was then evaluated in a mouse model.[Results]A strain SC-2 capable of inducing trained immunity in G.mellonella was identified as Enterococcus faecium.Administration of SC-2 significantly increased the percentages of neutrophils and macrophages in the peripheral blood and lung,decreased the bacteria loads in main organs,and increased the survival rate of mice infected by Streptococcus suis.[Conclusion]SC-2 had the effect of inducing trained immunity in mice.Our study provides new insights for immune regulation and disease treatment from the perspective of trained immunity.

关 键 词：屎肠球菌 驯化免疫 大蜡螟 分离鉴定 

分 类 号：S852.4[农业科学—基础兽医学]