分子检测技术在临床侵袭性曲霉病诊断中的应用进展  

Update of molecular diagnostic methods in invasive Aspergillosis

作　　者：杨双双[1] 黄世峰[1] 张传明[1] 张林博 曹炬[1] Yang Shuangshuang;Huang Shifeng;Zhang Chuanming;Zhang Linbo;Cao Ju(The First affiliated Hospital of Chongqing Medical University,the Surveillance Center of Fungal Diseases of Chongqing,Chongqing 400016,China)

机构地区：[1]重庆医科大学附属第一医院,重庆市真菌病监测网,重庆400016

出　　处：《中华检验医学杂志》2025年第2期297-302,共6页Chinese Journal of Laboratory Medicine

基　　金：国家自然科学基金(82070014);国家重点研发计划(2023YFC2412900);重庆市自然科学基金(cstc2021jcyj-msxmX0217);重庆市科卫联合项目(2022QNXM002)。

摘　　要：侵袭性曲霉病(IA)是机会致病性曲霉菌所致的严重丝状真菌感染性疾病。因发病隐匿、死亡率高,使得IA的精准诊断显得尤为迫切。随着分子诊断技术的发展,新的曲霉菌分子检测技术陆续报道,也逐渐应用于临床实验室真菌检测平台。鉴于此,本文总结了近年来曲霉菌核酸分子检测技术的临床应用及进展,探讨了其在IA诊断中的临床应用价值,以期为IA精准诊疗提供方法学选择依据,为IA体外诊断新技术研发提供参考。Invasive Aspergillosis(IA)is a severe filamentous fungal infection caused by opportunistic pathogenic Aspergillus.Due to its insidious incidence and high mortality rate,accurate diagnosis of IA is in urgent need.Recent advances in molecular diagnostic techniques have enabled novel approaches for Aspergillus detection in clinical fungal laboratory.To this end,this paper summarizes recent progress in molecular detection of Aspergillus nucleic acids and discusses its value in IA diagnosis.The findings provide guidance for both current diagnostic approaches and the development of new in vitro diagnostic technologies for IA.

关 键 词：曲霉菌病 聚合酶链反应 宏基因组测序 纳米孔测序 游离DNA 

分 类 号：R519[医药卫生—内科学]