成熟禾谷类作物种子胚乳细胞的显微CT扫描方法  

A New Cereal Seed Treatment Method for Displaying Endosperm Cell Structures Under Micro CT Scanning

作　　者：徐秀苹[1,2] 杨小雨 冯旻 Xiuping Xu;Xiaoyu Yang;Min Feng(Key Laboratory of Systematic and Evolutionary Botany,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China;China National Botanical Garden,Beijing 100093,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Guizhou Institute of Subtropical Crops,Guizhou Academy of Agricultural Sciences,Xingyi 562400,China)

机构地区：[1]中国科学院植物研究所,系统与进化植物学重点实验室,北京100093 [2]国家植物园,北京100093 [3]中国农业科学院作物科学研究所,北京100081 [4]贵州省农业科学院,贵州省亚热带作物研究所,兴义562400

出　　处：《植物学报》2025年第1期81-89,共9页Chinese Bulletin of Botany

基　　金：中国科学院技术支撑人才项目(2022);黔农科院青年科技基金(No.2021-20);黔农科院国基后补助(No.2021-12)。

摘　　要：禾谷类种子淀粉胚乳是人类主食的重要来源,但观察其成熟细胞形态的方法非常有限。显微CT是一种非破坏性的三维成像技术,是研究植物形态的有力工具。由于成熟禾谷类种子淀粉胚乳密度均匀,常规显微CT技术无法应用。在前期研究的基础上,用磷钨酸处理10种不同禾谷类作物种子,经CO_(2)临界点干燥后,再用显微CT扫描,可以清晰地分辨出禾谷类作物淀粉胚乳的细胞结构,为研究此类细胞结构提供了一种新方法。Cereal starch endosperm is the main source of human staple food,but the methods for observing its mature cell structure are not well developed.Micro CT technology,a non-destructive three-dimensional imaging technique,is a powerful tool for studying plant morphology.However,due to the uniform density of cereal starch endosperm,whose cell structures cannot be distinguished clearly by conventional micro CT techniques.In this study,we used phosphotungstic acid to treat ten different kinds of cereal seeds of seven crops,and then dried them by CO_(2) critical point dryer.We found that the cell structure of the treated endosperms can be clearly displayed by micro CT.Our method provides a new way for studying the structures and functions of cereal starch endosperm cells.

关 键 词：显微CT 禾谷类淀粉胚乳 磷钨酸 

分 类 号：S51[农业科学—作物学]