Biolog Eco解析不同抗性番木瓜土壤微生物群落特征  

Soil Microbial Community Characteristics of Different Resistant Papayas were Analyzed by Biolog Eco Microplates

作　　者：曹扬[1,2] 周霞 孔华[1,2] 霍姗姗 李春强[1] 谭燕华 CAO Yang;ZHOU Xia;KONG Hua;HUO Shanshan;LI Chunqiang;TAN Yanhua(Institute of Tropical Bioscience and Biotechnology,CATAS,Haikou,Hainan 571101,China;Sanya Research Institute,CATAS,Sanya,Hainan 572000,China)

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南海口571101 [2]中国热带农业科学院三亚研究院,海南三亚572000

出　　处：《热带农业科学》2025年第1期26-31,共6页Chinese Journal of Tropical Agriculture

基　　金：2020年海南省自然科学基金高层次人才项目(No.320RC716)。

摘　　要：番木瓜是重要的热带经济水果。由番木瓜环斑病毒(PRSV)引起的木瓜病害是木瓜属植物的主要病害之一,对番木瓜的产业造成了重大损失。采用对海南PRSV病毒具有田间抗性的番木瓜品种(HN)以及2种易感番木瓜品种(YK和NC)为研究材料,在番木瓜接种PRSV病毒前,采集抗性植株和易感植株的根际土壤,应用Biolog Eco微平板法,研究了不同抗性的番木瓜根际土壤微生物群落特征。结果表明:HN根际土壤微生物的AWCD值稍高于YK和NC,但差异不显著;土壤微生物群落的多样性指数分析显示,NC与HN以及YK的根际土壤微生物多样性指数均存在显著性差异,但HN与YK的根际土壤微生物多样性指数均不存在显著性差异。主成分分析表明,HN根际土壤微生物利用较多的碳源有7种:D-木糖/戊醛糖、L-精氨酸、吐温-40、衣康酸、1-磷酸葡萄糖、2-羟基苯甲酸、D-苹果酸;NC根际土壤微生物利用较多的碳源有5种:D-纤维二糖、N-乙酰基-D-葡萄糖胺、γ-羟丁酸、L-苏氨酸、腐胺;YK根际土壤微生物利用较高的碳源有4种:丙酮酸甲酯、i-赤藓糖醇、α-丁酮酸、L-天门冬酰胺。说明抗性番木瓜和易感番木瓜根际土壤微生物在这些碳源的利用能力上有特异性,长期种植可能导致微生物群落功能发生变化,从而使得番木瓜在病毒危害时表现出不同的抗性。Papaya(Carica papaya L.)is an important tropical economic fruit,and papaya ring spot virus(PRSV)is one of the primary diseases of papaya plants,causing significant losses to the papaya industry.In this study,papaya varieties with field resistance to PRSV(HN)and susceptible papaya varieties(YK and NC)were used as research materials.Rhizosphere soils from resistant and susceptible plants were collected before the papaya was inoculated with the PRSV virus,and soil microbial communities were studied via the Biolog Eco microplate method.The results revealed that the AWCD values of the HN rhizosphere soil microorganisms were slightly higher than those of susceptible papaya varieties YK and NC.Diversity index analysis of the rhizosphere soil microbial community revealed significant differences between NC and YK,as well as between NC and HN,but no significant differences were detected between HN and YK.Principal component analysis showed that there were seven carbon sources with higher utilization by HN soil microorganisms:D-xylose,L-arginine,Tween-40,itaconic acid,glucose-1-phosphate,2-hydroxybenzoic acid,and D-malic acid.There are five carbon sources with high utilization by NC soil microorganisms:D-cellobiose,N-acetyl-D-glucosamine,γ-hydroxybutyric acid,L-threonine,and putrescine.There were four carbon sources with higher utilization by YK soil microorganisms:pyruvic acid methyl ester,i-erythritol,α-ketobutyric acid,and L-asparagine.These results indicated that the rhizosphere soil microorganisms of resistant papaya and susceptible papayas were specific in utilizing these carbon sources,and long-term cultivation may lead to changes in the function of microbial communities,which may affect the resistance of papaya to virus damage.

关 键 词：番木瓜 土壤微生物群落 Biolog Eco 碳源利用 

分 类 号：S667.9[农业科学—果树学]