Both 20S and 19S proteasome components are essential for meiosis in male mice  

作　　者：Ting-Ting Han Li-Ying Wang Qiu-Xing Zhou Wei Wei Yan-Jie Ma Ying-Hong Chen Wei Li Zhen-Yu Ju Chao Liu 

机构地区：[1]Key Laboratory of Regenerative Medicine of Ministry of Education,Institute of Aging and Regenerative Medicine,Department of Developmental&Regenerative Medicine,College of Life Science and Technology,Jinan University,Guangzhou,Guangdong 510632,China [2]Guangzhou Women and Children’s Medical Center,Guangzhou Medical University,Guangzhou,Guangdong 510623,China

出　　处：《Zoological Research》2025年第1期27-40,共14页动物学研究(英文)

基　　金：supported by the National Science Fund for Distinguished Young Scholars (81925015);Science and Technology Project of Guangzhou (2023A03J0886,2023A03J0871);National Natural Science Foundation of China (82030039,32400709)。

摘　　要：The proteasome,an evolutionarily conserved proteolytic complex comprising the 20S core particle and 19S regulatory particles,performs both shared and distinct functions across various tissues and organs.Spermatogenesis,a highly complex developmental process,relies on proteasome activity at multiple stages to regulate protein turnover.In this study,we selected the 20S subunit PSMA1 and 19S regulatory subunit PSMD2 to investigate the potential functions of the proteasome in spermatogenesis.Using Psma1-EGFP and Psmd2-mCherry knock-in mouse models,we confirmed the expression of both subunits in all spermatogenic cell types,with pronounced presence in early germ cell development.To further clarify their functional significance,we specifically knocked out Psma1 and Psmd2 in germ cells.Deletion of either PSMA1 or PSMD2 led to disrupted spermatogenesis,characterized by the complete absence of sperm in the epididymis.Subsequent analysis indicated that loss of these proteasome components impaired meiotic initiation.Psma1 and Psmd2 knockout germ cells showed accumulation of DMRT1,a key regulator of mitosis-to-meiosis transition,leading to a reduction in STRA8 levels and consequent disruption of meiosis initiation.This study sheds light on the molecular mechanisms that govern meiotic initiation and identifies potential genes associated with male infertility.

关 键 词：PROTEASOME PSMA1 PSMD2 Meiotic initiation SPERMATOGENESIS 

分 类 号：Q492[生物学—生理学]