Immune Effects of the Triple Vaccine against Vibrio parahaemolyticus,Vibrio alginolyticus,and Edwardsiella tarda in Turbot(Scophthalmus maximus)  

副溶血弧菌、溶藻弧菌和迟缓爱德华菌三联疫苗对大菱鲆的免疫评价

作　　者：ZHANG Zhen-guo FENG Shou-ming 张振国;冯守明(天津市动物疫病预防控制中心,天津300402)

机构地区：[1]Tianjin Animal Disease Prevention and Control Center,Tianjin 300402,PRC

出　　处：《Agricultural Science & Technology》2024年第4期49-56,共8页农业科技与技术(英文版)

基　　金：天津科技计划项目(18ZXBFNC0030)。

摘　　要：This study aimed to evaluate the immune effects of the triple vaccine against Vibrio parahaemolyticus,Vibrio alginolyticus,and Edwardsiella tarda in turbot(Scophthalmus maximus).Turbot were immunized by oral administration,intraperitoneal injection,and immersion methods.Indirect ELISA was employed to measure the antibody levels in the serum,skin mucus,and hindgut mucosa.V.parahaemolyticus,V.alginolyticus,and E.tarda were used for challenge experiments,and the relative survival rate of turbot was determined.The results showed that the intraperitoneal injection group had the highest antibody titer in the serum.Antibodies were detected in the skin mucus only in the immersion group.The antibody titer in the hindgut mucosa was the highest in the oral administration group.The results of challenge experiments showed that the relative survival rate was 40%-50%in the intraperitoneal injection group and the immersion group,while it was 50%-60%in the oral administration group.The triple vaccine for oral administration was coated with acrylic resin,which prevented the digestion by gastric juice and enabled the release in the hindgut,effectively preventing turbot infections by V.parahaemolyticus,V.alginolyticus,and E.tarda.为了研究副溶血弧菌、溶藻弧菌和迟缓爱德华氏菌三联疫苗不同接种方式对大菱鲆的免疫效果,通过口服、腹腔注射和浸泡等免疫方式接种大菱鲆,采用间接ELISA测定血清、皮肤黏液和后肠黏膜中的抗体水平,分别用副溶血弧菌、溶藻弧菌和爱德华氏菌进行攻毒实验,测定大菱鲆的相对存活率。结果表明:在血清中,注射免疫组抗体含量最高;在皮肤黏液中,只有浸泡免疫组检测到抗体;在后肠黏膜中口服免疫组抗体最高。攻毒试验显示,注射免疫组和浸泡免疫组的相对存活率(RPS)为40%~50%,而口服RPS的相对存活率为50%~60%。该口服疫苗采用丙烯酸树脂包被,可阻止被胃液消化,可在后肠中释放,能有效预防大菱鲆受副溶血弧菌、溶藻弧菌和迟缓爱德华氏菌的感染。

关 键 词：Triple vaccine TURBOT Vibrio parahaemolyticus Vibrio alginolyticus Edwardsiella tarda 

分 类 号：S943[农业科学—水产养殖]