不同培养基配方对甘薯愈伤形成及生根的影响  

Effects of Different Medium Formulations on Callus Formation and Rooting of Sweet Potato

作　　者：范建新 邓仁菊 王维凤 FAN Jianxin;DENG Renju;WANG Weifeng(School of Life and Health Science,Kaili University,Kaili,Guizhou 556011;Guizhou Institute of Biotechnology,Guiyang,Guizhou 550006,China)

机构地区：[1]凯里学院大健康学院,贵州凯里556011 [2]贵州省生物技术研究所,贵州贵阳550006

出　　处：《贵州农业科学》2025年第2期22-28,共7页Guizhou Agricultural Sciences

基　　金：贵州省高等学校贵州山地特色园艺作物分子育种与品种创制重点实验室(黔教技〔2022〕053);凯里学院横向课题(HX202117);凯里学院博士启动项目(BS20220101);贵州省甘薯工程技术研究中心(黔科合平台人才〔2019〕5201)。

摘　　要：【目的】探明不同培养基配方对甘薯愈伤形成及生根的影响,为甘薯的组织培养提供技术参考。【方法】对紫云红芯薯、福薯24号、福薯404号等6个甘薯品种的叶片或茎段进行不同消毒方式处理、不同配方培养基培养,统计分析甘薯外植体的污染率、褐化率、出愈率、分化率及生根率,筛选适宜甘薯组织培养的外植体部位、消毒方法及各阶段适宜的培养基配方。【结果】甘薯叶片在0.1%HgCl_(2)灭菌8~10 min时,成活率高;茎段在0.1%HgCl_(2)灭菌12~15 min时,成活率高。甘薯叶片的愈伤组织诱导率显著高于茎段。在MS+NAA 0.1 mg/L+6-BA 0.5 mg/L培养基下,紫云红芯薯、福薯24号、福薯404号、渝薯27号、黔菜1号5个品种外植体的褐化率低,出愈率较高;普薯32号在MS+NAA 1.0 mg/L+6-BA 0.5 mg/L培养基下褐化率低,出愈率高。紫云红芯薯在MS+NAA 0.2 mg/L+6-BA 0.5 mg/L培养基中能够诱导分化,分化率1.67%;渝薯27号在MS+KT 2.0 mg/L+IAA 0.5 mg/L培养基中分化率为3.33%,其余4个品种的愈伤组织均未分化。6个甘薯品种在MS+6-BA 1.0 mg/L+IBA 0.3 mg/L培养基下生根率较高,其中,紫云红芯薯、普薯32号、渝薯27号的生根率均达100.0%。【结论】甘薯叶片的表面灭菌时间以0.1%的HgCl_(2)灭菌8~10 min为宜,茎段的表面灭菌时间以0.1%HgCl_(2)灭菌12~15 min为宜。甘薯叶片的愈伤组织诱导率显著高于茎段,且以MS+6-BA 1.0 mg/L+NAA 0.5 mg/L培养基的效果最好,出愈率均在97.8%以上。适宜甘薯不定芽生根的培养基为MS+6-BA 1.0 mg/L+IBA 0.3 mg/L,生根率均在92%以上。【Objective】The effects of different medium formulations on the callus formation and rooting of sweet potato were investigated to provide the technical references for tissue culture of sweet potato.【Method】The leaves or stem segments of six sweet potato varieties(Ziyun red core sweet potato,Fushu 24,Fushu 404,etc.)were treated with different disinfection methods and cultured on different medium formulations.The contamination rate,browning rate,callus induction rate,differentiation rate and rooting rate of sweet potato explants were statistically analyzed to screen suitable explant sites,disinfection methods,and suitable medium formulations for each stage of sweet potato tissue culture.【Result】The survival rate of leaves was high when sterilized with 0.1%HgCl_(2) for 8-10 minutes.The survival rate of stem segments was high when sterilized with 0.1%HgCl_(2) for 12-15 minutes.The induction rate of callus in sweet potato leaves was significantly higher than that in stem segments.Under MS+0.1 mg/L NAA+0.5 mg/L 6-BA medium,Ziyun red core sweet potato,Fushu 24,Fushu 404,Yushu 27 and Qiancai 1 had a low browning rate and high callus induction rate,while Pushu 32 had a same result under MS+1.0 mg/L NAA+0.5 mg/L 6-BA medium.Ziyun red core sweet potato could induce differentiation on MS+0.2 mg/L NAA+0.5 mg/L 6-BA medium,with the differentiation rate of 1.67%.Yushu 27 had a differentiation rate of 3.33%on MS+2.0 mg/L KT+0.5 mg/L IAA medium,the callus of other four varieties had not differentiated.The rooting rate of six sweet potato varieties on MS+1.0 mg/L 6-BA+0.3 mg/L IBA medium was high,among which the rooting rate of Ziyun red core sweet potato,Pushu 32 and Yushu 27 could reach 100.0%.【Conclusion】The optimal sterilization time for the surface of sweet potato leaves is 8-10 minutes with 0.1%HgCl_(2),while the optimal sterilization time for the surface of stem segments is 12-15 minutes with 0.1%HgCl_(2).The induction rate of callus in sweet potato leaves is significantly higher than that in stem segments,an

关 键 词：甘薯 培养基 MS NAA 6-BA 愈伤组织 生根 

分 类 号：S531[农业科学—作物学]