低纬高原不同种植模式紫色辣椒果实纤维素合成关键基因的挖掘  

Discovery of Key Genes Related to Cellulose Synthesis in Purple Chili Fruits Under Different Planting Modes in Low Latitude Plateau

作　　者：张丹丹 张雪廷 赵水灵 王绍祥 解志强 王文丽[1] 赵鹏宇 袁恩平 赵昶灵[1,4] ZHANG Dandan;ZHANG Xueting;ZHAO Shuiling;WANG Shaoxiang;XIE Zhiqiang;WANG Wenli;ZHAO Pengyu;YUAN Enping;ZHAO Changling(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming,Yunnan 650201;Institute of Vegetable Crops,Wenshan Zhuang and Miao Autonomous Prefecture Academy of Agricultural Sciences,Wenshan,Yunnan 663000;College of Ecological Technology and Engineering,Shanghai Institute of Technology,Shanghai 201418;Yunnan Institute of Plateau Organic Agriculture,Chuxiong,Yunnan 675099,China)

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]文山壮族苗族自治州农业科学院蔬菜研究所,云南文山663000 [3]上海应用技术大学生态技术与工程学院,上海201418 [4]云南高原有机农业研究院,云南楚雄675099

出　　处：《贵州农业科学》2025年第2期59-66,共8页Guizhou Agricultural Sciences

基　　金：文山壮族苗族自治州农业科学院资助项目“丘北辣椒花色苷合成对产量负效应的数字化表征”(HZ2021-01);云南省重大科技专项计划项目“云南特异辣椒资源高效利用研究”(202202AE090031)。

摘　　要：【目的】探明低纬高原生境中不同种植模式紫色辣椒果实纤维素合成相关的关键基因,为紫色辣椒果实纤维素合成机理研究及其种植模式选择提供依据。【方法】以紫色辣椒16HN01为试验材料,用分光光度法测定6个发育阶段果实的纤维素含量(CC),并基于转录组测序、qRT-PCR及相关性分析挖掘纤维素合成相关并应答种植模式的关键基因。【结果】果实6个发育阶段,大田种植和大棚种植16HN01果实的CC均逐渐增加,分别为3.79%~12.06%和2.35%~9.55%,大棚种植较大田种植显著降低17.90%~38.09%;大棚种植果实的β-谷甾醇葡糖基转移酶基因(SGT)、纤维素合酶基因(CesA2和CesA7)、类纤维素合酶基因(CSL-D3和CSL-G2)、NAM,ATAF1/2和CUC1/2基因(NAC)、油菜素内酯不敏感蛋白1-甲基磺酸乙酯-抑制子1基因(BES1)及v-myb鸟类成髓细胞性白血病病毒癌基因同源物48(MYB48)的转录水平分别较大田种植低17.13%、20.55%、17.73%、47.62%、33.68%、26.97%、42.00%和29.00%,Korrigan酶基因(Kor)的转录水平较大田种植高64.94%;大田种植CC含量与关键结构基因SG的CesA2、CSL-D3和CSL-G2呈显著正相关关系,相关系数分别为0.903、0.878和0.844,大棚种植CC含量与CesA2呈显著正相关关系,相关系数为0.854;大田种植CC含量与转录因子基因TF的NAC和BES1呈正相关关系,相关系数分别为0.560和0.413,大棚种植CC含量与NAC呈正相关关系,相关系数为0.671。【结论】低纬高原大田种植紫色辣椒果实纤维素合成相关的结构基因(SG)主要为CesA2、CSL-D3和CSL-G2,转录因子基因(TF)主要为NAC和BES1,大棚种植的SG主要为CesA2、CSL-G2和SGT,TF主要为NAC。大棚种植会导致紫色辣椒果实CC和纤维素合成相关基因(除Kor外)转录水平下降。【Objective】The key genes related to cellulose synthesis in purple chili fruits under two planting modes in the habitats of low latitude plateau were explored to provide a reference for studying the cellulose synthesis mechanism of purple chili fruits and selection of purple chili planting mode.【Method】The cellulose content of purple Capsicum annuum 16HN01 fruits was determined by spectrophotometry at six fruit development stages and the key genes related to cellulose synthesis and the planting mode were discovered by transcriptome sequencing,quantitative real-time fluorescence PCR(qRT-PCR)and correlation analysis.【Result】The cellulose content of purple C.annuum 16HN01 fruits in field and greenhouse modes gradually increased with fruit development process and was 3.79%-12.06%and 2.35%-9.55%respectively.The cellulose content of purple C.annuum 16HN01 fruits in greenhouse significantly decreased by 17.90%-38.09%compared with that in field.The transcriptional level of SGT,CesA2,CesA7,CSL-D3,CSL-G2,NAC,BES1 and MYB48 in purple C.annuum 16HN01 fruits planted in greenhouse was 17.13%,20.55%,17.73%,47.62%,33.68%,26.97%,42.00%and 29.00%lower than in field respectively.The transcriptional level of Korrigan gene(Kor)in purple C.annuum 16HN01 fruits planted in greenhouse was 64.94%higher than in field.The CC content of purple C.annuum 16HN01 fruits planted in field was significantly and positively related to CesA2,CSL-D3 and CSL-G2 and their correlation coefficients were 0.903,0.878 and 0.844 separately.The CC content of purple C.annuum 16HN01 fruits planted in greenhouse was positively related to CesA2 significantly and the correlation coefficients was 0.854.The CC content of purple C.annuum 16HN01 fruits planted in field was positively related to NAC and BES1 and their correlation coefficients were 0.560 and 0.413 respectively.The CC content of purple C.annuum 16HN01 fruits planted in greenhouse was positively related to NAC and the correlation coefficient was 0.671.【Conclusion】The structural gene(SG)and tr

关 键 词：低纬高原 种植模式 紫色辣椒 果实 纤维素合成相关关键基因 挖掘 

分 类 号：S641.3[农业科学—蔬菜学]