银屑病合并皮肤细菌感染病原菌和TLR/NF-κB信号通路水平  

Pathogenic bacteria distribution and TLR/NF-κB signaling pathways in patients with psoriasis complicated with cutaneous bacterial infection

作　　者：喻集保 裴凯旋 宁学思 黄肖肖 YU Jibao;PEI Kaixuan;NING Xuesi;HUANG Xiaoxiao(Linhai Traditional Chinese Medicine Hospital,Taizhou,Zhejiang317000,China)

机构地区：[1]临海市中医院皮肤科,浙江台州317000

出　　处：《中华医院感染学杂志》2025年第3期357-361,共5页Chinese Journal of Nosocomiology

基　　金：浙江省医药卫生科技计划基金资助项目(2021LY0469)。

摘　　要：目的探讨银屑病合并皮肤细菌感染患者病原菌和Toll样家族受体(TLR)/核因子κB(NF-κB)信号通路水平。方法选取2022年10月—2023年9月临海市中医院收治的300例银屑病患者作为研究对象,其中银屑病合并皮肤细菌感染患者设为感染组(97例),未合并皮肤感染患者设为未感染组(203例),另选取60名同期体检健康者作为健康组。分析银屑病合并皮肤细菌感染患者皮肤组织中病原菌特点;比较三组外周血TLR/N F-κB信号通路的表达;比较感染组与未感染组病情程度[银屑病面积严重程度指数(PASI)、皮损面积(BSA)];采用Pearson相关性分析法分析TLR/NF-κB信号通路表达与病情程度的相关性。结果97例银屑病合并皮肤细菌感染患者共培养分离病原菌103株,其中革兰阳性菌95株占92.23%,以金黄色葡萄球菌(83株,80.58%)为主;感染组与未感染组外周血TLRmRNA、NF-κB mRNA分别为(2.55±0.85)、(1.72±0.39)与(1.92±0.66)、(1.42±0.40)均高于健康组,其中感染组高于未感染组(P<0.05);感染组PASI、BSA评分分别为(18.79±4.91)、(14.05±2.68)分,均高于未感染组(P<0.05);Pearson相关性分析结果显示,外周血TLR mRNA、NF-κB mRNA表达水平均与PASI、BSA评分呈正相关(r=0.565,0.540,0.427,0.411,P均<0.05)。结论银屑病合并皮肤细菌感染患者病原菌主要为金黄色葡萄球菌,外周血TLR/NF-κB信号通路表达明显上调,并对银屑病患者合并皮肤细菌感染有一定的诊断价值。OBJECTIVE To investigate the distribution of pathogenic bacteria and the changes of Toll-like family receptor(TLR)/nuclear factor kB(κB)signaling pathway in patients with psoriasis complicated with cutaneous bacterial infection.METHODS Three hundred psoriasis patients admitted to Linhai Hospital of Traditional Chinese Medicine from Oct.2022 to Sep.2023 were selected as the study subjects,among which the patients with psoriasis combined with cutaneous bacterial infections were divided into the infected group(97 cases),and the patients without combined skin infections were divided into the uninfected group(203 cases),and other 60 healthy subjects during the same period were selected as the control group.The distribution characteristics of pathogenic bacteria in the skin tissues of patients with psoriasis combined with cutaneous bacterial infection were analyzed.The expression of TLR/κB signaling pathway in peripheral blood among three groups was compared.Psoriasis area severity index(PASI)and area of skin lesion(BSA)were compared between the infected and uninfected groups.The correlation between the expression of TLR/NF-κB signaling pathway and the severity of disease was analyzed by Pearson correlation analysis.RESULTS A total of 103 strains of pathogenic bacteria were co-cultured and isolated from 97 patients with psoriasis combined with skin bacterial infections,of which 95 strains of gram-positive bacteria accounted for 92.23%,and Staphylococcus aureus(83 strains,80.58%)was the main strain.TLR mRNA andκB mRNA in peripheral blood of the infected and uninfected groups were(2.55±0.85),(1.72±0.39),(1.92±0.66)and(1.42±0.40),respectively,higher than those of control group,and the indicators above in the infected group were higher than those in the non-infected group(P<0.05).The PASI and BSA scores in infected group were(18.79±4.91)points and(14.05±2.68)points,higher than those in uninfected group(P<0.05).Pearson correlation analysis showed that the expression levels of TLR mRNA andκB mRNA in peripheral blo

关 键 词：银屑病 皮肤细菌感染 病原菌 Toll样家族受体 核因子ΚB 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]