一套无抓捕应激的实验狨猴椅设计及其初步应用  

Design of a Capture Stress-Free Marmoset Monkey Chair Device for Experiments and Its Preliminary Application

作　　者：徐聖业 黄俊锋 陈一航 常亮堂 XU Shengye;HUANG Junfeng;CHEN Yihang;CHANG Liangtang(Laboratory Animal Center,Center for Excellence in Brain Science and Intelligence Technology,Chinese Academy of Sciences,Shanghai 200031,China;School of Basic Medical Sciences,Nanjing Medical University,Nanjing 210029,China;Lingang Laboratory,Shanghai 201602,China)

机构地区：[1]中国科学院脑科学与智能技术卓越创新中心实验动物中心,上海200031 [2]南京医科大学基础医学院,南京210029 [3]临港实验室,上海201602

出　　处：《实验动物与比较医学》2025年第1期67-72,共6页Laboratory Animal and Comparative Medicine

摘　　要：目的为避免实验猴因直接抓捕引发应激反应的问题,提高狨猴在行为学、双光子成像和电生理等实验中的适应性和实验效率,研制一种无须抓捕即可将狨猴保定的实验猴椅装置。方法通过3D图形设计和打印,制作一套可在狨猴实验时配合使用的转运笼和猴椅。首先将转运笼与实验饲养笼的食盆出口对接,轻微驱赶狨猴进入转运笼,之后将转运笼与猴椅连接,再次轻微驱赶狨猴进入猴椅,保定后进行后续实验。通过观察转运和保定的时间效率、狨猴的配合度以及应激反应等进行有效性测试和改进。结果经过测试与改进,该装置可以在无须抓捕的情况下,顺利完成狨猴的保定,显著提高了实验的流畅度和效率。随着操作次数的增加,狨猴会更加配合,操作速度加快,实验效率得到了显著提升。使用该装置后,实验狨猴的应激反应明显减少。特别是与传统的抓捕方法相比,使用该装置能显著减少狨猴的焦虑和不适,提高了其在实验中的配合度。结论本团队设计的猴椅装置能够在无须抓捕的情况下轻松实现狨猴的保定,在确保后续实验顺利进行的同时,还能保障动物福利。该装置具有操作简便、实用性强、成本低等优点,易于推广使用。Objective To avoid stress responses in experimental monkeys caused by direct capture,and to improve the adaptability and experimental efficiency of marmosets in behavioral,two-photon imaging,and electrophysiological experiments,a device for immobilizing marmosets without the need for capture is developed.Methods A set of compatible transport cage and monkey chair was produced through 3D graphic design and printing.First,the transport cage was aligned with the feeding outlet of the experimental housing cage,and the marmoset was gently guided into the transport cage.Then,the transport cage was connected to the monkey chair,and the marmoset was gently guided into the chair for immobilization.Subsequent experiments were carried out afterward.The effectiveness was evaluated by observing the efficiency of transport and immobilization,the marmoset cooperation level,and stress responses.Results After testing and improvements,the device successfully completed immobilization of marmosets without the need for capture,significantly improving the fluency and efficiency of the experiment.As the number of operations increased,the marmosets became more cooperative,and the operation speed was significantly enhanced.After using the device,the stress responses were noticeably reduced,with marmosets showing lower stress levels.In particular,compared to traditional capture methods,the use of this device significantly reduced marmoset anxiety and discomfort,increasing their cooperation levels during the experiment.Conclusion The monkey chair device designed allows for restraint of marmosets without the need for capture,ensuring smooth progress of subsequent experiments while also safeguarding animal welfare.This device is easy to operate,highly practical,cost-effective,and has great potential for widespread application.

关 键 词：普通狨猴 猴椅 保定 应激 

分 类 号：Q95-33[生物学—动物学] Q-337