CRSwNP中医证型与TFH细胞相关因子的相关性研究  

Study on correlation between traditional Chinese medicine syndrome types and TFH cell related factors in CRSwNP

作　　者：王奥维 时文杰 WANG Aowei;SHI Wenjie(Graduate Shool of Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Department of Otorhinolaryngology&Head and Neck Surgery Affiliated Haikou Hospital of Xiangya Medical College,Central South University,Haikou 570208,Hainan,China)

机构地区：[1]天津中医药大学研究生院,天津301617 [2]中南大学湘雅医学院附属海口医院耳鼻咽喉头颈外科,海南海口570208

出　　处：《山东大学耳鼻喉眼学报》2025年第1期54-60,共7页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：海南省卫生健康行业科研项目(21A200438)。

摘　　要：目的探讨免疫球蛋白E(immunoglobulin E,IgE)、滤泡辅助性T细胞(follicular helper T cell,TFH)相关因子白细胞介素21(interleukin-21,IL-21)与慢性鼻窦炎伴鼻息肉(chronic rhinosinusitis with nasal polyps,CRSwNP)中医证型的关系变化。方法回顾性研究行鼻内镜鼻窦手术的58例CRSwNP患者,分为寒湿凝聚证组和湿热蕴积证组,采集患者一般资料、血清总IgE以及中医四诊资料。术中收集鼻息肉组织标本,并采用酶联免疫吸附测定法检测患者鼻息肉组织的IgE及IL-21,免疫组化法检测患者鼻息肉组织Bcl-6阳性表达。运用相关统计学方法,探讨TFH细胞及相关因子表达与中医证型的相关性。结果两中医证型患者的鼻息肉组织IgE、IL-21、Bcl-6水平差异均无统计学意义(P均>0.05);寒湿凝聚证患者鼻息肉Bcl-6表达与IgE水平呈正相关(r=0.491,P=0.024),湿热蕴积证患者鼻息肉Bcl-6表达与IgE水平未见明显相关性(P=0.195);寒湿凝聚证患者血清总IgE水平明显高于湿热蕴积证患者(P=0.013)。结论鼻息肉中IgE、IL-21、Bcl-6的表达与中医证型无关,血清IgE与中医证型相关。寒湿凝聚证组Bcl-6对鼻息肉组织IgE的影响大于湿热蕴积证组。Objective To explore the relationship between immunoglobulin E(IgE),follicular helper T-cell(TFH)related factor interleukin-21(IL-21),and traditional Chinese medicine syndrome types of chronic rhinosinusitis with nasal polyps(CRSwNP).Methods A retrospective study was conducted on 58 patients with CRSwNP who underwent endoscopic sinus surgery were divided into a cold dampness condensation syndrome group and a dampness heat accumulation syndrome group.General information,total serum igand traditional Chinese medicine diagnostic data were collected;collect nasal polyp tissue samples during surgery,and use enzyme-linked immunosorbent assay to detect IgE and IL-21 in the patient's nasal polyp tissue.Use immunohistochemistry to detect the positive expression of Bcl-6 in the patient's nasal polyp tissue.Using relevant statistical methods,explore the correlation between the expression of TFH cells and related factors and the types of traditional Chinese medicine syndromes.Results There was no statistically significant difference in the levels of IgE,IL-21,and Bcl-6 in nasal polyp tissue between the two traditional Chinese medicine syndrome types(all P>0.05);There is a positive correlation between Bcl-6 expression and IgE levels in nasal polyps of patients with cold dampness condensation syndrome(r=0.491,P=0.024),There is no correlation between IgE levels and Bcl-6 expression in nasal polyps of patients with dampness heat accumulation syndrome(P=0.195);The total serum IgE levels in patients with cold dampness aggregation syndrome were significantly higher than those in patients with dampness heat accumulation syndrome(P=0.013).Conclusion The expression of IgE,IL-21,and Bcl-6 in nasal polyps is not related to traditional Chinese medicine syndrome types,while serum IgE is related to traditional Chinese medicine syndrome types.In the cold dampness accumulation syndrome group,the effect of Bcl-6 on IgE in nasal polyp tissue is greater than that in the heat dampness accumulation syndrome group.

关 键 词：慢性鼻窦炎 鼻息肉 滤泡辅助性T细胞 中医证型 

分 类 号：R765.4[医药卫生—耳鼻咽喉科]