不锈钢载体型过氧化氢生物指示剂芽孢回收影响因素研究  

Influencing factors of spore recovery of stainless steel carrier hydrogen peroxide biological indicators

作　　者：薛琪 戴佳晟 周亚倩 童锴 孙巍群 XUE Qi;DAI Jia-sheng;ZHOU Ya-qian;TONG Kai;SUN Wei-qun(Zhejiang Tailin Medical Engineering Co.Ltd.,Hangzhou 311400,China)

机构地区：[1]浙江泰林医学工程有限公司,浙江杭州311400

出　　处：《中国消毒学杂志》2025年第2期87-89,共3页Chinese Journal of Disinfection

摘　　要：目的 研究稀释液种类、浸泡时间和超声时间对不锈钢载体型过氧化氢生物指示剂嗜热脂肪地芽孢杆菌芽孢计数结果的影响。方法 将嗜热脂肪地芽孢杆菌芽孢用水、生理盐水、0.1%吐温80水溶液和0.1%吐温80的生理盐水4种稀释液浸泡2 h,然后超声40 min,筛选芽孢回收率最高的稀释液,用此稀释液在超声0~80 min和浸泡0~4 h不同组合条件下,筛选芽孢回收率最高的超声时间和浸泡时间,每个组合设3个平行样本,计算芽孢回收率。结果 以0.1%吐温80水溶液为稀释液,芽孢回收率最高,达到118.25%。40 kHz超声频率、200W功率下超声40 min芽孢回收率为105.25%,均高于其他超声时间。浸泡2 h后的芽孢回收率最高,达到91.9%。结论 0.1%吐温80水溶液浸泡2 h,超声40 min进行芽孢计数,生物指示剂芽孢回收率最高。Objective To study the effects of diluent type,soaking time and ultrasonic time on the spore count of Geobacillus stearothermophilus of stainless steel hydrogen peroxide bioindicator.Methods Geobacillus stearothermophilus spores biological indicator were soaked in water,normal saline,0.1%Tween 80 aqueous solution and 0.1%Tween 80 saline for 2 h,and then sonicated for 40 min to screen the dilution with the highest spore recovery.The ultrasonic time and soaking time with the highest spore recovery were screened under different combination conditions of ultrasonic 0~80 min and soaking 0~4 h,and three parallel samples were set up for each combination to calculate the spore recovery rate.Results With 0.1%Tween 80 aqueous solution as dilution,the spore recovery rate was the highest,reaching 118.25%.The spore recovery rate of 40 kHz ultrasound frequency and 40 min ultrasound at 200 W power was 105.25%,which were higher than those of other ultrasound times.After soaking for 2 h,the spore recovery rate was the highest,reaching 91.9%.Conclusion After soaking in 0.1%Tween 80 aqueous solution for 2 h and ultrasonic for 40 min for spore counting,the spore recovery rate of biological indicator is the highest,reaching 118.25%.

关 键 词：生物指示剂 芽孢回收率 过氧化氢 洗脱 

分 类 号：R187[医药卫生—流行病学]