电感耦合等离子体质谱法测定不同产地构树叶22种无机元素及健康风险评估  

Determination of 22 Inorganic Elements in Broussonetia papyrifera Leaves from Different Habitats and Health Risk Assessment by ICP-MS

作　　者：马鹏程 梁卜文 申国华 董培智 项新华[3] MA Pengcheng;Liang Buwen;Shen Guohua;DONG Peizhi;XIANG Xinhua(Shanxi University of Chinese Medicine;Shanxi Key Laboratory of Food and Drug Safety Prevention and Control,Institute of Drug Inspection Technology,Inspection and Testing Center of Shanxi Province;National Institutes for Food and Drug Control)

机构地区：[1]山西中医药大学,山西晋中030619 [2]山西省检验检测中心药品检验技术研究所食品药品安全防控山西省重点实验室,太原030031 [3]中国食品药品检定研究院,北京102629

出　　处：《中国药学杂志》2025年第3期234-243,共10页Chinese Pharmaceutical Journal

基　　金：食品药品安全防控山西省重点实验室项目资助(202204010931006)。

摘　　要：目的建立同时测定构树叶中22种无机元素含量的电感耦合等离子体质谱(ICP-MS)联用方法,分析不同产地构树叶无机元素的差异性,并对重金属及有害元素的安全性和健康风险进行评估,为构树叶的质量评价与资源的有效开发提供参考。方法构树叶微波消解后,22种元素以其相对分子质量相近的元素为内标,采用ICP-MS法测定,进行方法学考察后,测定样品,利用正交偏最小二乘法(OPLS-DA)比较不同产地无机元素的差异性,以单项污染指数(Pi)和综合污染指数(Pc)进行安全性评价,并通过计算每日摄入的最大重金属量(EDI)、靶标危害系数(THQ)和致癌风险(CR)对其进行健康风险评估。结果22种元素测定的线性关系良好,相关系数r^(2)≥0.991,精密度、稳定性、重复性试验相对标准差(RSD)值均符合分析要求,各元素的检出限在0.0006~1.6873μg·L^(-1)之间,回收率在83.63%~106.58%之间。测定不同产地的24批构树叶样品,构树叶中元素K、Ca、Mg、P的含量较高,分别为19098、5258、4882、2904mg·kg^(-1)。主成分分析得到5个主因子,共筛选出Co、Al、Fe、Ni、Sr、Mg、K为构树叶的主要特征性元素。Pi和Pc均为优良安全等级,EDI和CR结果表明,构树叶重金属及有害元素对人体健康没有潜在的风险,但THQ提示构树叶样品中的As元素可能对人体健康有影响。结论构树叶中含有丰富的无机元素,重金属及有害元素对人体健康影响较小,人体所必需的K、Ca、Mg、Fe、Na、Zn等元素的含量均较高。此方法灵敏度高、快速、准确,可用于构树叶无机元素的定量分析,对其无机元素的研究具有重要价值。OBJECTIVE To establish an inductively coupled plasma mass spectrometry(ICP-MS)method for the simultaneous determination of 22 kinds inorganic elements in Broussonetia papyrifera leaves,analyze the differences of inorganic elements in leaves from different origins,and evaluate the safety and health risks of heavy metals and harmful elements,so as to provide a reference for the quality evaluation of Broussonetia papyrifera leaves and the effective development of resources.METHODS After microwave digestion of Broussonetia papyrifera leaves,22 elements were analyzed by ICP-MS using Relative molecular mass similar elements as internal standards.After methodological investigations,samples were analyzed,and the orthogonal partial least squares discriminant analysis(OPLS-DA)method was utilized to compare variability of inorganic elements from diverse sources.Safety was evaluated by individual pollution index(Pi)and comprehensive pollution index(Pc),while health risk was assessed by calculating maximum daily intake of heavy metals(EDI),target hazard quotient(THQ),and carcinogenic risk(CR).RESULTS The linearity of the 22 elements determined was excellent with a correlation coefficient r^(2)≥0.991.The relative standard deviation(RSD)values of precision,stability,and reproducibility tests met analytical requirements.The detection limit of each element was between 0.0006 to 1.6873μg·L^(-1),and recovery rate was between 83.63%and 106.58%.The contents of K,Ca,Mg,and P in 24 batches of leaf samples from different origin were higher,which were 19098,5258,4882 and 2904 mg·kg^(-1),respectively.Principal component analysis revealed 5 key factors,identifying Co,Al,Fe,Ni,Sr,Mg,and K as the main characteristic elements of Broussonetia papyrifera leaves.Pi and Pc had excellent safety ratings.EDI and CR results indicated no potential health risks from heavy metals and harmful elements in Broussonetia papyrifera leaves,but THQ suggests that the as element in the leaves may have an impact on human health.CONCLUSION The Broussonetia pa

关 键 词：构树叶 电感耦合等离子体质谱 无机元素 微波消解法 健康风险评估 

分 类 号：R282[医药卫生—中药学]