基于UPLC-QE-Orbitrap-MS/MS的骨康胶囊化学成分分析  

Analysis of Chemical Constituents of Gukang Capsules by UPLC-QE-Orbitrap-MS/MS

作　　者：许瑛婕 黄鸿运 汪洋 刘春花[1] 郑林[3] 孙佳[3] 李勇军[1,2,3] XU Yingjie;HUANG Hongyun;WANG Yang;LIU Chunhua;ZHENG Lin;SUN Jia;LI Yongjun(State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University Engineering Research Center for the Development and Application of Ethnic Medicine and TCM(Ministry of Education,Guiyang 550004,China;College of Pharmacy,Guizhou Medical University,Guiyang 550004,China;Guizhou Provincial Key Laboratory of Pharmaceutics,National Engineering Research Center of Miao′s Medicines,Guizhou Medical University,Guiyang 550004,China)

机构地区：[1]贵州医科大学民族药与中药开发应用教育部工程研究中心,省部共建药用植物功效与利用国家重点实验室,贵阳550004 [2]贵州医科大学药学院,贵阳550004 [3]贵州医科大学,贵州省药物制剂重点实验室,国家苗药工程技术研究中心,贵阳550004

出　　处：《中国药学杂志》2025年第3期290-301,共12页Chinese Pharmaceutical Journal

基　　金：贵州省科技计划项目资助(黔科合中引地〔2023〕006);贵州省高层次创新型人才培养计划项目资助(〔2016〕5677);贵阳市科技计划项目资助(筑科合同〔2024〕2-35)。

摘　　要：目的采用超高效液相色谱-四极杆静电场轨道阱高分辨质谱(UPLC-QE-Orbitrap-MS/MS)技术分析骨康胶囊的化学成分。方法该药物用体积分数70%甲醇提取液采用HypersilGOLDC_(18)(2.1mm×100mm,1.9μm)色谱柱,以0.1%甲酸水溶液-乙腈为流动相梯度洗脱,流速为0.3mL·min^(-1),柱温45℃,进样量1μL。采用电喷雾离子源(ESI),在正、负离子同时扫描模式下采集骨康胶囊的质谱数据,利用软件结合对照品比对、数据库匹配、文献报道及质谱规律对骨康胶囊化学成分进行分析鉴定。结果从骨康胶囊中共鉴定出137个化合物,主要包括黄酮类、皂苷类、有机酸类、香豆素类等,其中27个化合物通过对照品比对鉴定,均明确药材来源归属。结论该方法快速、高效地分析出骨康胶囊的主要化学成分,为其药效物质基础及质量控制的后续研究奠定基础。OBJECTIVE To study the chemical composition of Gukang Capsules by ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitwell high resolution mass spectrometry(UPLC-QE-Orbitrap-MS/MS).METHODS The analysis of 70%methanol extract of the drug was performed on a 45℃thermostatic Hypersil GOLD C_(18)column(2.1 mm×100 mm,1.9μm)with mobile phase consisting of 0.1%formic acid solution-acetonitrile gradiently eluted at 0.3 mL·min^(-1),and the injection volume was 1μL.The information of the chemical constituents of Gukang Capsules was acquired in positive and negative ion modes by electrospray ion source,and the chemical composition was analyzed and identified by software combined with control comparison,database matching,literature reports and mass spectrometry rules.RESULTS A total of 137 compounds were identified from Gukang Capsules,mainly including flavonoids,saponins,organic acids and coumarins,etc.,among which 27 compounds were identified by comparison with reference substances and their origins of medicinal materials were determined.CONCLUSION In this study,the main chemical composition of Gukang Capsules was identified by this rapid and efficient analytic method,which laid a foundation for further research on pharmacodynamic substance base and quality control.

关 键 词：骨康胶囊 超高效液相色谱-四极杆静电场轨道阱高分辨质谱 黄酮类 皂苷类 

分 类 号：R917[医药卫生—药物分析学]