基于UHPLC-Q-TOF-MS技术分析暗紫贝母花的化学成分  

Analysis of Chemical Constituents in Flower of Fritillaria unibracteata P.K.Hsiao et K.C.Hsia by UHPLC-Q-TOF-MS

作　　者：任奕洁 耿昭 何成军 钟恋 苟琰 高必兴 蒲婧哲 张亚中 张志淳 李小红[1] REN Yijie;GENG Zhao;HE Chengjun;ZHONG Lian;GOU Yan;GAO Bixing;PU Jingzhe;ZHANG Yazhong;ZHANG Zhichun;Li Xiaohong(College of Food and Biological Engineering,Chengdu University,Chengdu 610106,China;MIIT Public Service Platforms for Industrial Technological Base,NMPA Key Laboratory for Quality Evaluation of Traditional Chinese Medicine,Traditional Chinese Patent Medicine,Sichuan Institute for Drug Control(Sichuan Testing Center of Medical Devices),Chengdu 611731,China;NMPA Key Laboratory for Quality Research and Evaluation of Traditional Chinese Medicine,Anhui Institute for Food and Drug Control,Hefei 230051,China)

机构地区：[1]成都大学食品与生物工程学院,成都610106 [2]四川省药品检验研究院(四川省医疗器械检测中心),国家药品监督管理局中成药质量评价重点实验室,工业和信息化部产业技术基础公共服务平台,成都611731 [3]安徽省食品药品检验研究院,国家药品监督管理局中药质量研究与评价重点实验室,合肥230051

出　　处：《中国药学杂志》2025年第1期32-38,共7页Chinese Pharmaceutical Journal

基　　金：国家药品监督管理局中药质量研究与评价重点实验室开放课题资助(AHYJ-KFKT-202401)。

摘　　要：目的为充分利用川贝母资源,本研究通过超高效液相色谱-四极杆飞行时间质谱联用技术(UHPLC-Q-TOF-MS)鉴定暗紫贝母花中化学成分。方法采用Thermo Hypersil GOLD aQ(2.1 mm×150 mm,1.9μm)色谱柱,0.1%甲酸水溶液-甲醇梯度洗脱,流速0.3 mL·min^(-1),柱温35℃,采用电喷雾电离源(ESI),正、负离子模式分别扫描。结果通过对照品比对、一级质谱精确测定相对分子质量、二级质谱碎片信息,结合文献报道,从暗紫贝母花中鉴定了71个化学成分,包括生物碱24个,黄酮12个,氨基酸9个等,并鉴定出贝母素甲、贝母素乙等有效成分。结论本研究运用UHPLC-Q-TOF-MS技术对其化学组成进行了详尽解析,结果表明暗紫贝母花存在潜在的药用价值,研究结论为其资源的合理开发与可持续利用提供了科学依据。OBJECTIVE To identify the chemical constituents in flower of Fritillaria unibracteata P.K.Hsiao et K.C.Hsia(F-F,Chinese:Anzibeimu Hua)by employing ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UHPLC-Q-TOF-MS)for the optimal utilization of Fritillaria cirrhosa D.Don(Chinese:Chuanbeimu)resources.METHODS The separation was conducted on Thermo Hypersil GOLD aQ column(2.1 mm×150 mm,1.9μm)eluted with methanol-0.1%formic acid in a gradient mode at a flow rate of 0.3 mL·min^(-1),the column temperature was maintained at 35℃,and positive and negative electrospray ionization(ESI)was adopted for determine the chromatographic effluents.RESULTS Through comparison with reference standards,precise molecular weight determination from primary mass spectrometry,and analysis of fragmentation patterns from secondary mass spectrometry,combined with existing literature,71 chemical constituents were identified in F-F.These identified compounds encompass 24 alkaloids,12 flavonoids,9 amino acids,and additional substances,including the notable detection of peimine and peiminine within F-F.CONCLUSION This study employs UHPLC-Q-TOF-MS technology to conduct an exhaustive analysis of the chemical composition of F-F.From the results,it can be seen that the F-F possess potential medicinal value.This study provides scientific basis for the rational development and sustainable utilization of its resources.

关 键 词：暗紫贝母花 超高效液相色谱-四极杆飞行时间质谱联用 成分鉴定 裂解规律 

分 类 号：R284[医药卫生—中药学]