彝族医药灵通清肺汤通过调控c-Fos表达缓解内毒素介导的大鼠肺毛细血管渗漏  

Yi nationality medicine Lingtong Qingfei Decoction improving lipopolysaccharide-mediated pulmonary capillary leakage in rats via regulating c-Fos expression

作　　者：吴伟 吴俊汐 耿福昌 刘彬 赵珊 范青峰 尹洪寅 杨怡 Wu Wei;Wu Junxi;Geng Fuchang;Liu Bin;Zhao Shan;Fan Qingfeng;Yin Hongyin;Yang Yi(Research Institute of Medicine and Herbs of Yi Nationality&Gastroenterology Department,Liangshan Prefecture Integrated Traditional and Western Medicine Hospital,Xichang 615000;Good Doctor Pharmaceutical Group Co,Ltd.Chengdu 610073;School of Medical and Life Science,Chengdu University of TCM,Chengdu 610000)

机构地区：[1]凉山州中西医结合医院,彝族医药研究所&消化内科,西昌615000 [2]好医生药业集团,成都610073 [3]成都中医药大学,医学与生命科学学院,成都610000

出　　处：《中国抗生素杂志》2025年第1期112-119,共8页Chinese Journal of Antibiotics

基　　金：四川省中医药管理局中医药科研专项资助(No.2021MS036)。

摘　　要：目的研究彝医药灵通清肺汤(Lingtong Qingfei Decoction,LTQFD)改善脂多糖(lipopolysaccharide,LPS)介导大鼠肺毛细血管渗漏(capillary leakage,CL)的潜在分子机制。方法用LTQFD干预LPS诱导的急性肺损伤(acute lung injury,ALI)大鼠,进行肺组织基因芯片分析及蛋白互作网络构建,检测肺炎性状态、CL指标和气道指标、呼吸功能和病理改变;进而用正常大鼠LTQFD含药血清预处理LPS干预的大鼠肺静脉内皮细胞(rat pulmonary vein endothelial cells,RPPVECs),检测促炎细胞因子释放、跨膜电阻和细胞旁渗漏及枢纽蛋白c-Fos的表达。结果LTQFD抑制了LPS介导的Toll样受体级联激活和c-Fos高表达,改善了过激的炎性反应,缓解了肺和RPPVECs的CL,从而改善了ALI的肺病理改变、气道指标和呼吸功能。结论LTQFD可能通过逆转LPS介导的Toll样受体级联激活和c-Fos高表达来改善ALI肺CL、病理学改变、气道指标和呼吸功能。Objective To investigate the potential molecular mechanism of Lingtong Qingfei Decoction(LTQFD)in ameliorating lipopolysaccharide(LPS)-induced pulmonary capillary leakage in rats.Methods Rats with LPS-induced acute lung injury(ALI)were treated with LTQFD.Lung tissue gene chip analysis and the construction of a protein interaction network were conducted.Subsequently,assessments were made on pneumonia status,capillary leakage,airway indicators,respiratory function,and pathological changes.LPS-intervened rat pulmonary vein endothelial cells(RPPVECs)were pre-treatment with LTQFD(normal rat serum containing medication)to evaluate pro-inflammatory cytokine release,transepithelial electrical resistance,paracellular leakage,and hub protein c-Fos expression.Results LTQFD inhibited LPS-mediated Toll-like receptor cascade activation and high expression of c-Fos,improved the excessive inflammatory response,relieved capillary leakage in the lungs and RPPVECs,and thus improved lung pathological changes,airway indicators,and respiratory function in ALI(P<0.05).Conclusion LTQFD might improve pulmonary capillary leakage,pathological changes,airway indicators,and respiratory function in ALI by reversing LPS-mediated Toll-like receptor cascade activation and high expression of c-Fos.

关 键 词：灵通清肺汤 彝族药物 急性肺损伤 毛细血管渗漏 炎性反应 C-FOS 

分 类 号：R966[医药卫生—药理学]