睾丸类器官培养技术的进展  

Advances in testicular organoid culture technology

作　　者：黄明昊 杜国尧 吴登龙[2] 乐威[2] Huang Minghao;Du Guoyao;Wu Denglong;Le Wei(Tongji University School of Medicine,Shanghai200092,China;Department of Urology,Tongji Hospital of Tongji University,Shanghai200065,China)

机构地区：[1]同济大学医学院,上海200092 [2]同济大学附属同济医院泌尿外科,上海200065

出　　处：《中华生殖与避孕杂志》2025年第1期96-102,共7页Chinese Journal of Reproduction and Contraception

基　　金：同济大学原创基础探索研究项目(22120240358)。

摘　　要：随着男性生殖系统疾病和生育障碍发病率的增加,男性不育的诊治及生育力保存成为需要临床克服的难题。类器官是一种利用成体干细胞或胚胎干细胞在体外不同微环境中培养的细胞产物,具备和天然组织类似的组织解剖和生理功能。近年来,睾丸类器官在体外诱导精子增殖、分化等方面的技术有所突破,为解决上述问题提供了新的思路。本文对常见的睾丸类器官技术(微孔培养法、微流体培养法、三层梯度系统、气-液平面培养法、细胞外基质水凝胶支架、悬滴培养法、3D打印技术)进行了简要介绍,包括技术特点、研究情况和技术瓶颈等,并简要概述了部分常见的类器官培养液在睾丸类器官领域应用的特点和应用情况。With the increasing incidence of male reproductive system diseases and reproductive dysfunctions,diagnosis and treatment of male infertility and fertility preservation have become clinical challenges to be overcome.Organoids,a kind of cell product cultured in vitro under different microenvironments by using adult stem cells or embryonic stem cell,can possess histological anatomy and physiological function that are similar to natural tissues.In recent years,technological breakthroughs have been made in testicular organoids in terms of sperm proliferation and differentiation induced in vitro,providing a new idea for solving the above problems.This article briefly introduces common testicular organoid techniques(micropore culture,microfluidics culture method,three-layer gradient system,air-liquid interface culture,extracellular matrix hydrogel scaffold,hanging drop cultures,and 3D printing technology,etc.),including technical features,research conditions,technical bottlenecks and summarizes the application characteristics and situations of some ordinary organoid culture solutions in the field of testicular organoids as well.

关 键 词：睾丸 类器官 生物支架 3D培养 

分 类 号：R698.2[医药卫生—泌尿科学]